TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken  

作　　者：CUI Huan-xian LUO Na GUO Li-ping LIU Lu XING Si-yuan ZHAO Gui-ping WEN Jie 

机构地区：[1]State Key Laboratory of Animal Nutrition/Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第3期853-863,共11页农业科学学报（英文版）

基　　金：funded by the grants from the National Natural Science Foundation of China(31872340);the State Key Laboratory of Animal Nutrition(2004DA125184G2109);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-IAS04);the earmarked fund for China Agriculture Research System(CARS-41)。

摘　　要：The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2(TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated(P<0.01) in muscle tissue of individuals with high intramuscular fat content.Weighted gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat,C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism(such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction(such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated(P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat content.Our findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway.

关 键 词：TIMP2 ECM intramuscular adipocytes muscle satellite cells CHICKEN 

分 类 号：S852.2[农业科学—基础兽医学]