超高效液相色谱-四级杆飞行时间串联质谱分析白背叶全成分  

Analysis of the Whole Composition of Mallotus apelta by Ultra Performance Liquid Chromatography-quadrupole Time-of-flight Tandem Mass Spectrometry

作　　者：麦琬婷 章波 陆建媚 覃裕翠 苏晓丹 黄秋洁 叶勇[1,5] MAI Wanting;ZHANG Bo;LU Jianmei;QIN Yucui;SU Xiaodan;HUANG Qiujie;YE Yong(Pharmaceutical College,Guangxi Medical University,Nanning 530021,China;Guigang People's Hospital,Guigang 537199,China;Department of Experimental Center,Guilin Medical College,Guilin 541001,China;Pharmaceutical College,Guangxi University of Chinese Medicine,Nanning 530001,China;Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,南宁530021 [2]贵港市人民医院药剂科,贵港537199 [3]桂林医学院科学实验中心,桂林541001 [4]广西中医药大学药学院,南宁530001 [5]广西生物活性分子研究与评价重点实验室,南宁530021

出　　处：《医药导报》2023年第4期529-535,共7页Herald of Medicine

基　　金：国家自然科学基金资助项目(81360689);广西自然科学基金资助项目(2022GXNSFDA035063,2018GXNSFAA050078,2015GXNSFAA139173);广西高校中青年教师科研基础能力提升基金资助项目(2019KY0148,2019KY0315)。

摘　　要：目的使用超高效液相色谱-四级杆飞行时间串联质谱(UHPLC-Q-TOF-MS/MS)方法鉴定白背叶叶提取物中的化学成分。方法通过UHPLC-Q-TOF-MS/MS获取其裂解信息,结合二级质谱数据库、Scifinder数据库等,结合Progenesis SDF Studio软件、Progenesis QI软件和搜索文献进行MS和MS/MS碎片解析。结果鉴定出63个化学成分,包括21个有机酸类、19个黄酮类、6个酚类、4个酯类、4个醇类、3个生物碱类、2个糖类、2个醛类、1个胆碱类及1个喹啉类,所鉴定出成分的准确质量测量结果均为±5 ppm误差。结论通过该方法对白背叶叶提取物中的化学成分进行全面的分析,为白背叶药材化学成分分析提供基础。Objective To identify the chemical constituents of the leaves of Mallotus apelta using ultra high performance liquid chromatography coupled with quadruple time of flight mass spectrometry(UHPLC-Q-TOF-MS/MS).Methods The fragmentation information was obtained by UHPLC-Q-TOF-MS/MS,combined with secondary mass spectrometry database,Scifinder database,etc.Combined with Progenesis SDF Studio software,Progenesis QI software and literature search,MS and MS/MS fragment analysis were carried out.Results A total of 63 chemical components were identified,including 21 organic acids,19 flavonoids,6 phenols,4 esters,4 alcohols,3 alkaloids,2 sugars,2 aldehydes,1 choline,and 1 quinoline,and the accurate mass measurement results of the identified components are all±5 ppm error.Conclusion This method provides a comprehensive analysis of the chemical constituents of the leaves of Mallotus apelta,which provides a basis of the analysis for the chemical constituents of medicinal materials of the Mallotus apelta.

关 键 词：白背叶 超高效液相色谱-四级杆飞行时间串联质谱 成分分析 

分 类 号：R917[医药卫生—药物分析学]