维生素D3对新生大鼠坏死性小肠结肠炎的肠道保护作用研究  

Study on the intestinal protective effect of Vitamin D3 on necrotizing enterocolitis in neonatal rats

作　　者：孙乐科 胡琳凤 柯波[1] SUN Leke;HU Linfeng;KE Bo(Neonatal Department,Jiangxi Provincial People’s Hospital,The First Affiliated Hospital of Nanchang Medical College,Nanchang,330006,China)

机构地区：[1]江西省人民医院,南昌医学院第一附属医院新生儿科,南昌330006

出　　处：《江西医药》2023年第1期12-15,19,共5页Jiangxi Medical Journal

基　　金：江西省卫建委普通科技计划项目,编号20195051;江西省自然科学基金项目,编号20192BAB205019。

摘　　要：目的建立坏死性小肠结肠炎(NEC)新生大鼠模型并研究维生素D3干预对肠道保护作用。方法采用人工代乳品、缺氧和冷应激的方法诱导新生大鼠NEC,干预组提前给予维生素D3干预。HE病理和TUNEL染色检测肠管组织病理变化和细胞凋亡,ELISA实验和免疫组化染色分别检测肠道组织中炎症因子和凋亡蛋白的表达水平。结果病理结果显示维生素D3干预可明显减少NEC新生大鼠肠道凋亡细胞数量,减轻组织损伤;ELISA和免疫组化染色结果显示维生素D3干预可降低肠道组织炎症因子TNF-α的表达水平,还降低Cleaved-Caspase3、iNOS和COX2表达水平。结论维生素D3干预可降低NEC大鼠肠道组织炎症因子的表达,抑制细胞凋亡,发挥肠道保护作用。Objective To established a neonatal rat model of necrotizing enterocolitis(NEC)and study the protective effect of Vitamin D3 intervention on intestinal.Methods The NEC animal models were established by methods of artificial rearing,hypoxia and cold stress;Rats in the intervention groups were pretreated with Vitamin D3.HE and TUNEL staining were performed to analyze the pathological changes and apoptotic epithelial cells in the ileum;Enzyme-linked immunosorbent assay(ELISA)and immunohistochemical staining was preformed to analysize the expression levels of inflammatory factors and protein associated in apoptosis.Results Histopathologic examination shown that Vitamin D intervention can reduce the inflammatory factor TNF-αin intestinal tissue and also reduce the expression levels of Cleaved-Caspase3,iNOS and COX2.Conclusion Vitamin D3 intervention can reduce the expression of inflammatory factors and inhibit apoptosis in intestinal tissues of NEC rats,and play a role in intestinal protection.

关 键 词：坏死性小肠结肠炎 新生大鼠 维生素D3 肠道保护 炎症因子 

分 类 号：R722.1[医药卫生—儿科]