SLE患者SR-BⅠ受体胆固醇外流功能的变化及作用机制  

Changes and mechanism of SR-BⅠreceptor cholesterol efflux function in SLE patients

作　　者：朱星星 寇艳婷 原江水[3] 宋卫青[3] ZHU Xingxing;KOU Yanting;YUAN Jiangshui;SONG Weiqing(Qingdao Medical College,Qingdao University,Qingdao,Shandong 266073,China;Graduate School of Dalian Medical University,Dalian,Liaoning 116044,China;Department of Laboratory,Qingdao Municipal Hospital,Qingdao,Shandong 266071,China)

机构地区：[1]青岛大学青岛医学院,山东青岛266073 [2]大连医科大学研究生院,辽宁大连116044 [3]山东省青岛市市立医院检验科,266071

出　　处：《重庆医学》2023年第6期814-819,共6页Chongqing medicine

基　　金：国家自然科学基金青年基金项目(31800618)。

摘　　要：目的探讨清道夫受体B类Ⅰ型(SR-BⅠ)介导的胆固醇外流途径在系统性红斑狼疮(SLE)患者中的变化及临床意义。方法选取2017年1月至2021年10月在青岛市市立医院就诊的门诊或住院60例SLE患者作为SLE组,选取同期性别、年龄相匹配的51例健康体检者为对照组。回顾性收集两组临床资料,构建SR-BⅠ慢病毒RNA干扰重组载体,选取测序正确的质粒进行病毒包装后,用慢病毒感染CHO细胞抑制SR-BⅠ表达;以去除载脂蛋白(Apo)B的主要含高密度脂蛋白(HDL)的血浆为胆固醇接受体诱导CHO细胞胆固醇外流,计算胆固醇外流率,分析SLE患者SR-BⅠ受体介导的胆固醇外流途径发生的变化。结果与对照组比较,SLE组预测动脉粥样硬化及发生心血管风险的相关指标——非高密度脂蛋白胆固醇(Non HDL-C)、动脉粥样硬化指数(AI)、血浆动脉粥样指数(AIP)及脂蛋白结合指数(LCI)明显升高(P<0.05);与SLE未合并心血管疾病患者比较,SLE合并心血管疾病患者甘油三酯(TG)、AI及LCI明显升高(P<0.05);与CHO细胞比较,各组SR-BI抑制表达的CHO(CHO/SR-BⅠ)细胞胆固醇外流率明显升高,差异有统计学意义(P<0.05)。与对照组比较,SLE组CHO、CHO/SR-BⅠ细胞胆固醇外流率明显升高,差异有统计学意义(P<0.05)。SLE合并与未合并心血管疾病患者CHO、CHO/SR-BⅠ细胞胆固醇外流率比较,差异无统计学意义(P>0.05)。结论SR-BⅠ介导的胆固醇外流途径在SLE患者中发生明显变化,这可能与SLE患者中心血管合并症高发具有相关性。Objective To investigate the changes and clinical significance of cholesterol efflux pathway mediated by scavenger receptor class B typeⅠ(SR-BⅠ)in patients with systemic lupus erythematosus.Methods A total of 60 patients diagnosed with SLE who were hospitalized or out-patient in Qingdao Municipal Hospital from January 2017 to October 2021 selected as the SLE group,51 healthy people matched for gender and age were selected as the control group.Clinical data were collected retrospectively.The recombinant vector of SR-BⅠlentiviral RNA interference was constructed,and the correctly sequenced plasmids was selected for virus packaging,the expression of SR-BⅠwas inhibited by infecting CHO cells with lentivirus.Cholesterol efflux of CHO cells was induced by removing Apolipoprotein(Apo B)-containing high-density lipoprotein(HDL)plasma as cholesterol recipients.Cholesterol efflux rate was calculated to analyze the changes of SR-BⅠreceptor-mediated cholesterol efflux pathway in SLE patients.Results Compared with the control group,non-high density lipoprotein cholesterol(Non-HDL-C),atherosclerotic index(AI),plasma atherosclerotic index(AIP),and lipoprotein binding index(LCI)in the SLE group were significantly higher(P<0.05).Compared with the SLE group without cardiovascular disease,triglycerides(TG),AI and LCI in the SLE group with cardiovascular disease were significantly higher(P<0.05).Compared with CHO cells,the cholesterol efflux rate of CHO cells inhibited expression by SR-BⅠ(CHO/SR-BⅠcells)was significantly increased in each group,with statistical significance(P<0.05).Compared with the control group,the cholesterol efflux rate of CHO and CHO/SR-BⅠcells in the SLE group was significantly increased,the difference was statistically significant(P<0.05).There was no significant difference in the cholesterol effluence rate of CHO and CHO/SR-BⅠcells between the SLE group withe or without cardiovascular disease(P>0.05).Conclusion The SR-BⅠ-mediated cholesterol efflux pathway has obvious changes in SLE patie

关 键 词：系统性红斑狼疮 胆固醇外流 动脉粥样硬化 清道夫受体B类Ⅰ型 高密度脂蛋白 

分 类 号：R593.24[医药卫生—内科学]