小球藻多糖对人树突状细胞成熟的作用和机制研究  

Study on the effect and mechanism of polysaccharide from Chlorella on maturation of human dendritic cells

作　　者：梁镕伊 袁清霞 赵龙岩 张丽峰 肖健 Liang Rongyi;Yuan Qingxia;Zhao Longyan;Zhang Lifeng;Xiao Jian(Institute of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China;Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学基础医学院,南宁530200 [2]广西中医药大学广西高发传染病中西医结合转化医学重点实验室,南宁530200 [3]广西中医药大学海洋药物研究院,南宁530200

出　　处：《广西医科大学学报》2023年第2期228-235,共8页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81860780);广西自然科学基金资助项目(No.2020GXNSFAA297162);广西研究生教育创新计划项目(No.YCSW2021216)。

摘　　要：目的:探讨小球藻多糖(PFC)对人树突状细胞(DC)的成熟作用及其相关机制的初步研究。方法:使用纯化后的PFC对外周血单核细胞来源的不成熟DC进行干预,使用流式细胞术检测PFC干预后DC表面CD83和CD86表达变化,利用酶联免疫法(ELISA)检测细胞因子IL-6、TNF-α和IL-10水平的变化;继而通过比较PFC处理组和对照组的转录谱基因表达差异及通路富集分析,寻找其作用途径,并使用相关分子通路抑制剂进行初步验证。结果:PFC可上调DC表面CD83和CD86的表达,促进DC细胞因子IL-10的释放;生信分析结果提示PFC可能通过Toll样受体2/4(TLR2/4)发挥作用,利用TLR2和TLR4通路抑制剂下调了PFC诱导的DC CD86表达。其中TLR4抑制剂干预后CD86表达水平显著下降(P<0.05)。结论:PFC可能通过TLR2和TLR4通路上调人外周血单核细胞来源DC表面标志物CD83和CD86表达水平的提高,从而促进DC成熟。Objective:To explore the effect of polysaccharides from Chlorella(PFC)on the maturation of human dendritic cells(DC)and its related mechanism.Methods:The immature DC derived from peripheral blood mononuclear cells was interfered with with purified PFCS.Flow cytometry was used to detect the expression changes of CD83 and CD86 on the DC surface after the intervention of PFCS,and the cytokine levels of IL-6,TNF-αand IL-10 were detected by enzyme-linked immunosorbent assay(ELISA).Then,the transcriptional gene expression difference and pathway enrichment analysis between the PFC treatment group and the control group were compared to find the action pathway,and the related molecular pathway inhibitors were used for preliminary verification.Results:PFC could up-regurate the expressions of CD83 and CD86 on the surface of DC and promote the release of IL-10.The results of bioinformatics analysis showed that PFC might play a role through Toll-like receptor 2/4(TLR2/4).TLR2 and TLR4 pathway inhibitors were used to down-regulate the PFC-induced expression of CD86 in DC.The expression level of CD86 significantly decreased after TLR4 inhibitor intervention(P<0.05).Conclusion:PFCS may up-regulate the expression levels of DC surface markers:CD83and CD86 derived from human peripheral blood mononuclear cells through TLR2 and TLR4 pathways,thus promoting DC maturation.

关 键 词：小球藻多糖 人树突状细胞 成熟 转录组 

分 类 号：R96[医药卫生—药理学]