HPLC法测定六个地区泡囊草生品、奶制及浸膏中山莨菪碱、莨菪碱及东莨菪碱的含量  

Determination of anisodamine,hyoscyamine and scopolamine in raw,milk and extract of Physochlaina phsaloides in six regions by HPLC

作　　者：月英 侯亚星 齐力格尔 包欣欣 包明兰[1,2] 敖民 YUE Ying;HOU Ya-xing;Qiliger(School of Mongolian Medicine,Inner Mongolia Minzu University,Tongliao 028000,China;不详)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]蒙医药研发工程教育部重点实验室,内蒙古通辽028000 [3]内蒙古库伦蒙药有限公司,内蒙古库伦028200

出　　处：《中国处方药》2023年第3期38-42,共5页Journal of China Prescription Drug

基　　金：内蒙古自治区人民政府蒙医药标准化项目(2019—〔MB018〕);内蒙古自然科学基金项(2019MS08173);内蒙古民族大学中药学(蒙药学)重点学科建设项目(ZYX003);《内蒙古民族大学蒙药炮制研究科研创新团队》项目(MYY009);内蒙古自治区2019年“双一流”专项-“蒙药炮制学创新团队建设”项目(MY20190004);蒙药研发国家地方联合工程研究中心开放基金(MDK2019038);内蒙古民族大学关于2023年度自治区直属高校基本科研业务费项目-科研创新平台能力建设支持计划(蒙药炮制解毒增效机制研究平台)(MYY256)。

摘　　要：目的建立高效液相色谱(HPLC)测定六个地区所产泡囊草中山莨菪碱、莨菪碱、东莨菪碱的含量。方法采用色谱柱为Agilent Venusil MP C18柱(300 mm×4.6 mm,5μm),以0.1%磷酸水(A)-乙腈(B)为流动相进行梯度洗脱,检测波长为210 nm,流速1 ml/min,柱温35℃,进样量10μl。结果山莨菪碱在1.13~3.39μg/ml内与色谱峰面积呈良好的线性关系(r=0.9991),平均加样回收率为97.21%,RSD为1.46%;莨菪碱在1.34~4.02μg/ml内与色谱峰面积呈良好的线性关系(r=0.9994),平均加样回收率为96.31%,RSD为0.97%;东莨菪碱在1.63~4.89μg/ml内与色谱峰面积呈良好的线性关系(r=0.9994),平均加样回收率为98.52%,RSD为0.83%。结论该文建立的HPLC测定方法,主峰分离效果明显,方法简便,重复性好,可作为泡囊草中山莨菪碱、莨菪碱、东莨菪碱含量的测定方法。Objective To establish a high performance liquid chromatography(HPLC)method for the determination of anisodamine,hyoscyamine and scopolamine in Physochlaina phsaloides from six regions.Methods HPLC method was used with a Agilent Venusil MP C 18(4.6 mm×300 mm,5μm)column.0.1%Phosphoric acid wate(r A)-Acetonitrile(B)solution was used as mobile phase forgradient elution,the flow rate was 1.0 ml·min^(-1),the column temperature was 35℃,the injection volume was 10μl,and the detection wavelength was 210 nm.Results The range of anisodamine was 1.13~3.39μg/ml(r=0.9991).The average recoveries were 97.21%and RSD was 1.46%.The range of scopolamine was 1.34~4.02μg/ml(r=0.9994),and the average recovery was 96.31%with RSD of 0.97%.The range of scopolamine was 1.63~4.89μg/ml,and the linear relationship with the peak area was good(r=0.9994).The average recoveries were 98.52%and RSD was 0.83%.Conclusion The HPLC method established in this paper can be used for the determination of anisedamine,hyoscyamine and scopolamine in Physochlaina phsaloides.The main peak separation effect is obvious,the method is simple and reproducible.

关 键 词：泡囊草 含量测定 山莨菪碱 莨菪碱 东莨菪碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]