基于太赫兹时域光谱技术的名贵动物药材龟甲的真伪品鉴别  

Authenticity identification of famous and precious animal medicine tortoiseshell by terahertz time-domain spectroscopy

作　　者：蒋俊 曾仪晨[2] 陈芷莹 裴丹 何津津 唐海发 曹云飞 蔡辉 JIANG Jun;ZENG Yichen;CHEN Zhiying;PEI Dan;HE Jinjin;TANG Haifa;CAO Yunfei;CAI Hui(Department of Integrated Chinese and Western Medicine,General Hospital of Eastern Theater Command of PLA,Nanjing Jiangsu 210002;School of Pharmacy,Jiangsu University,Zhenjiang Jiangsu 212013;Zhenjiang Food and Drug Supervision and Inspection Center,Zhenjiang Jiangsu 212000;Yangzijiang Pharmaceutical Group Co.LTD,Taizhou Jiangsu 225321,China)

机构地区：[1]中国人民解放军东部战区总医院中西医结合科,江苏南京210002 [2]江苏大学药学院,江苏镇江212013 [3]镇江市食品药品监督检验中心,江苏镇江212000 [4]扬子江药业集团有限公司,江苏泰州225321

出　　处：《江苏大学学报（医学版）》2023年第2期167-173,共7页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81703773);江苏省自然科学基金资助项目(BK20170560);镇江市重点研发计划项目(SH2022075);江苏大学大学生创新创业重点项目(202110299137Y);镇江市第六期169工程科研项目。

摘　　要：目的:建立一种利用太赫兹时域光谱和多种化学计量学方法鉴别龟甲真伪的新方法。方法:选用正品乌龟和伪品红耳彩龟的腹甲进行实验,先用经典的性状鉴别、显微鉴别方法比较两种样品的典型性状特征,后利用太赫兹时域光谱仪测得样品的光谱信息,对样品谱图进行平滑处理和基线校准,并运用化学计量方法进行数据分析以区分正品和伪品龟甲。结果:性状鉴别结果显示完整正、伪品龟甲样品仅有细微差异,且其碎片或粉末基本无差异;显微鉴别未在两种样品中发现明显的特征差异性结构;两种样品的太赫兹时域光谱曲线图经快速傅里叶变换滤波器平滑滤波处理、正交偏最小二乘法判别分析处理后呈现显著差异。结论:利用太赫兹时域光谱技术结合正交偏最小二乘法判别分析可以实现龟甲真伪品的精准鉴别。Objective:To establish a new method for identification of true and false tortoiseshells by terahertz time-domain spectroscopy and stoichiometry.Methods:The samples of authentic tortoiseshell and fake tortoiseshell from the red-eared slider were selected for further experiments.The typical characteristics of the two samples were compared by classical character identification and microscope identification methods,and then the spectral information of the samples was measured by terahertz time-domain spectrometer.The sample spectra were smooth-processed and baseline calibrated,and the data were analyzed by stoichiometric method to distinguish the authentic tortoiseshells from the fake tortoiseshells.Results:The results of character identification showed that there were only slight differences between intact and fake tortoiseshell samples,and little differences in fragments or powder.Microscopic identification of the two samples did not find obvious characteristic difference structure.After fast Fourier transform filter and orthogonal partial least square discriminant analysis,the terahertz time-domain spectral curves of the two samples showed significant differences.Conclusion:Terahertz time-domain spectroscopy combined with orthogonal partial least square discriminant analysis could achieve accurate identification of true and false tortoiseshells.

关 键 词：太赫兹时域光谱 快速傅里叶变换 龟甲 正交偏最小二乘法判别分析 真伪鉴别 动物药 

分 类 号：R284.1[医药卫生—中药学]