山药块茎膨大期淀粉积累及淀粉合成相关基因表达分析  

Analysis of starch accumulation and starch synthesis-related gene expression during tuber expansion stage of yam

作　　者：索宁宁 张艳芳[1] 高圆丽 赵令敏 葛明然 刘杰才[1] 霍秀文[1] SUO Ningning;ZHANG Yanfang;GAO Yuanli;ZHAO Lingmin;GE Mingran;LIU Jiecai;HUO Xiuwen(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010019,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010019

出　　处：《中国瓜菜》2023年第3期69-76,共8页China Cucurbits And Vegetables

基　　金：国家自然科学基金(31860558);内蒙古自治区科技计划项目(2020GG0044)。

摘　　要：为探讨山药块茎膨大期淀粉的积累机制,以大和长芋山药为试验材料,测定了块茎膨大期各类淀粉含量、淀粉合成关键酶活性以及淀粉合成相关基因的表达水平。结果表明,山药块茎膨大期总淀粉、直链淀粉及支链淀粉的积累呈先增加后降低的趋势,种植后150 d支链淀粉占总淀粉含量的88.35%,总淀粉含量的增加主要是支链淀粉的积累;ADP-葡萄糖焦磷酸化酶(AGPase)、淀粉分支酶(SBE)与支链淀粉的积累呈极显著正相关,是直接参与支链淀粉积累的重要功能酶。AGPase、SSⅡ、SSⅢ、SBEⅡ基因的表达量与支链淀粉含量呈极显著正相关,是支链淀粉积累的关键因素。SBE对淀粉积累表现为直接正效应,AGPase和束缚态淀粉合成酶(GBSS)在直链淀粉积累中体现为直接正效应。这些研究对揭示山药块茎淀粉合成的分子机制具有重要意义。In order to explore the accumulation mechanism of starch during the expansion stage in yam tubers,we investigated the changes in starch content of various types,activity of key enzymes in starch synthesis and gene expression patterns of starch synthesis key enzymes in Dahechangyu yam tubers.The results showed that the accumulation of total starch,amylose and amylopectin showed a trend of increasing first and then decreasing during tuber expansion stage of yam,and amylopectin accounts for 88.35%of the total starch content 150 days after planting.The increase of total starch content mainly results from amylopectin accumulation.Glucose-1-phosphate adenylyltransferase(AGPase)and starch-branching enzyme(SBE)have significant positive correlation with the accumulation of amylopectin,and are important functional enzymes directly involved in the accumulation of amylopectin.The gene expression level of AGPase,SSII,SSIII,SBEII was positively correlated with amylopectin content,which was the key factor of amylopectin accumulation in yam tubers.SBE showed a directly positive effect on starch accumulation,AGPase and granule bound starch synthase have directly positive effect on amylose accumulation.These studies are of great significance to reveal the molecular mechanism of starch synthesis in yam tubers.

关 键 词：山药块茎 淀粉积累 淀粉合成关键酶 基因表达 

分 类 号：S632.1[农业科学—蔬菜学]