中试SBR处理鸭场沼液过程中脱氮除碳效能及微生物群落演替  

Performance of a pilot-scale sequential batch reactor(SBR)on nitrogen and carbon removals and its characteristics of microbial community succession from biogas slurry from duck farm

作　　者：文红平 杨小明 成郁楠 刘梦雪 罗子锋 李强 李永涛[1,2,3] 张振 WEN Hongping;YANG Xiaoming;CHENG Yunan;LIU Mengxue;LUO Zifeng;LI Qiang;LI Yongtao;ZHANG Zhen(College of Natural Resources and Environment,Joint Institute for Environmental Research&Education,South ChinaAgricultural University;Wens Foodstuff Group Co.,Ltd.;Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs)

机构地区：[1]华南农业大学资源环境学院,中英环境科学研究中心 [2]温氏食品集团股份有限公司 [3]农业农村部环境保护科研监测所

出　　处：《环境工程技术学报》2023年第2期669-678,共10页Journal of Environmental Engineering Technology

基　　金：2022年乡村振兴战略专项资金省级项目(440000220000000035282);温氏股份科技重点项目(WENS-2020-1-ZDHB-006)。

摘　　要：通过在鸭场搭建中试规模的序批式反应器(SBR),以稀释鸭场沼液作为进水,并用蔗糖调节进水COD,评估SBR处理鸭场沼液过程中的脱氮除碳效能和微生物群落演替。结果表明:阶段Ⅰ(1~20 d)为污泥接种及水质适应阶段,进水碳氮比(C/N)小于2,COD和NH_(4)^(+)-N浓度约为200 mg/L,COD和NH_(4)^(+)-N去除率在第8天分别达到80%和90%;阶段Ⅱ(21~55 d)为系统稳定运行阶段,进水C/N小于2,COD和NH_(4)^(+)-N浓度分别为200~500、200~400 mg/L,COD去除率约为60%,NH_(4)^(+)-N去除率超过80%;阶段Ⅲ(56~95 d)为模拟有机物浓度变化阶段,进水C/N为1.2~5.5,COD和NH_(4)^(+)-N浓度分别为300~1 400、150~400 mg/L,COD和NH_(4)^(+)-N的去除率均大于80%,同时发现低温是SBR脱氮除碳的主要限制因素之一。通过微生物16S rRNA全长测序发现,Proteobacteria和Gammaproteobacteria分别为系统中门和纲水平下的优势微生物菌群。从属水平分析,试验期间系统内微生物发生了明显演替,在运行稳定后均形成了具有脱氮除碳功能的优势微生物群落。表明SBR可以实现对低C/N鸭场沼液的高效脱氮除碳,对高NH_(4)^(+)-N浓度和低C/N的鸭场沼液具有较好的应用潜力。A pilot-scale sequential batch reactor(SBR)was built in the duck farm,the duck farm biogas slurry was diluted as the influent,and the influent COD was regulated with sucrose,to evaluate the efficiency of nitrogen and carbon removal and the microbial community succession in the process of SBR treating the biogas slurry of the duck farm.The results showed that StageⅠ(1-20 d)was the sludge inoculation and water quality adaptation stage,in which the influent C/N was controlled to be less than 2,and the concentrations of COD and NH_(4)^(+)-N were around 200 mg/L.It was found that the removal efficiencies of COD and NH_(4)^(+)-N rapidly increased to 80%and 90%,respectively,within the first 8th day.StageⅡ(21-55 d)was the stabilization stage,in which C/N of the influent was also less than 2,and the concentrations of COD and NH_(4)^(+)-N were 200-500 and 200-400 mg/L,respectively.In this stage,the removal efficiencies of COD were fluctuated around 60%,while the removal efficiencies of NH_(4)^(+)-N were more than 80%.StageⅢ(56-95 d)was the simulation stage for organic concentration change,in which C/N of the influent was in the range of 1.2 to 5.5,while the influent COD and NH_(4)^(+)-N concentrations were 300-1400 and 150-400 mg/L,respectively,and the removal rate of COD and NH_(4)^(+)-N was greater than 80%in this stage.Meanwhile,low temperature was one of the main limiting factors for SBR nitrogen and carbon removal.Full-length sequencing of 16S rRNA from microorganisms revealed that Proteobacteria and Gammaproteobacteria were the dominant microbial flora at the phylum and class levels in the system,respectively.A significant shift of microbial community in terms of genus level was detected.The dominant microbial community species with nitrogen and carbon removal functions were formed after the operation stabilization.In general,SBR could achieve efficient nitrogen and carbon removal and had promising application potential in the real practice of treating biogas slurry from duck farm with a characteristics of high

关 键 词：鸭场沼液 中试规模 SBR 脱氮除碳 微生物群落演替 

分 类 号：X703[环境科学与工程—环境工程]