Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-basedmulti-material hydrogel composites  

作　　者：Suihong Liu Haiguang Zhang Tilman Ahlfeld David Kilian Yakui Liu Michael Gelinsky Qingxi Hu 

机构地区：[1]Centre for Translational Bone,Joint and Soft Tissue Research,Faculty of Medicine and University Hospital Carl Gustav Carus,Technische Universität Dresden,01307 Dresden,Germany [2]Rapid Manufacturing Engineering Center,School of Mechatronic Engineering and Automation,Shanghai University,Shanghai 200444,China [3]Shanghai Key Laboratory of Intelligent Manufacturing and Robotics,School of Mechatronic Engineering and Automation,Shanghai University,Shanghai 200444,China [4]National Demonstration Center for Experimental Engineering Training Education,Shanghai University,Shanghai 200444,China

出　　处：《Bio-Design and Manufacturing》2023年第2期150-173,共24页生物设计与制造（英文）

基　　金：The authors acknowledge the funding support from the National Natural Science Foundation of China(Nos.52175474 and 51775324);the China Scholarship Council(No.202006890054).

摘　　要：Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to the precise spatial control over depositing the biomaterial.Despite their widespread utilization and numerous advantages,the development of suitable novel biomaterials for extrusion-based 3D printing of scaffolds that support cell attachment,proliferation,and vascularization remains a challenge.Multi-material composite hydrogels present incredible potential in this field.Thus,in this work,a multi-material composite hydrogel with a promising formulation of chitosan/gelatin functionalized with egg white was developed,which provides good printability and shape fidelity.In addition,a series of comparative analyses of different crosslinking agents and processes based on tripolyphosphate(TPP),genipin(GP),and glutaraldehyde(GTA)were investigated and compared to select the ideal crosslinking strategy to enhance the physicochemical and biological properties of the fabricated scaffolds.All of the results indicate that the composite hydrogel and the resulting scaffolds utilizing TPP crosslinking have great potential in tissue engineering,especially for supporting neo-vessel growth into the scaffold and promoting angiogenesis within engineered tissues.

关 键 词：Multi-material composite hydrogel Crosslinking mechanism CHITOSAN GELATIN Egg white 3D printing 

分 类 号：TB33[一般工业技术—材料科学与工程]