联合接种原生动物和木霉对黄瓜生长及土壤微生物区系的影响  

Effects of protists and Trichoderma co-inoculation on cucumber growth and soil microbial communities

作　　者：莫雅旎 郭赛 欧燕楠 焦子轩 黄若菱 李荣[1] 沈宗专[1] Geisen Stefan 沈其荣[1] MO Yani;GUO Sai;OU Yannan;JIAO Zixuan;HUANG Ruoling;LI Rong;SHEN Zongzhuan;Geisen Stefan;SHEN Qirong(College of Resources and Environmental Sciences/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/Educational Ministry Engineering Center of Resource-Saving Fertilizers,Nanjing Agricultural University,Nanjing 210095,China;Wageningen University,6708PB Wageningen,The Netherlands)

机构地区：[1]南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化研究重点实验室/江苏省有机固体废弃物协同创新中心/教育部资源节约型肥料工程技术研究中心,江苏南京210095 [2]瓦赫宁根大学,荷兰瓦赫宁根6708PB

出　　处：《南京农业大学学报》2023年第2期297-305,共9页Journal of Nanjing Agricultural University

基　　金：江苏省自然科学基金青年基金项目(BK20200544);中央高校基本科研业务费专项资金(XUEKEN2022004);江苏省政策引导类计划(国际科技合作/港澳台科技合作)项目(BZ2020026);大学生创新训练计划项目(202110307122Y)。

摘　　要：[目的]本文旨在探究吞噬性原生动物、真菌及两者联合对黄瓜生长和土壤微生物群落组成的影响。[方法]供试黄瓜品种为‘露丰黄瓜’,采用盆栽试验,设计不接种对照(CK)、单独接种贵州木霉(Trichoderma guizhouense NJAU 4742)(T处理)、单独接种丝竹虫(Cercomomnas lenta)(C处理)、组合接种丝竹虫和贵州木霉(T+C处理)4个处理,处理2周后采集植株及土壤样品,评估各处理对黄瓜性状及土壤细菌和真菌群落的影响。[结果]相较于CK,T、C和T+C 3个处理黄瓜株高分别增加6.0%、12.5%和21.5%,生物量分别提高5.6%、12.9%和24.0%,即贵州木霉和丝竹虫均能促进黄瓜生长且两者组合能够进一步增强促生效应。基于主坐标分析发现,3个处理均显著改变了土壤细菌和真菌群落组成,随机森林模型进一步发现细菌和真菌群落组成是影响黄瓜生物量的主要因素。线性判别分析发现,与CK相比,3个处理分别激发22、51和82个关键细菌操作分类单元(OTU),2、6和16个关键真菌OTU;T+C处理激发了更多的与黄瓜促生相关的OTU,其中,有21个关键细菌OTU仅在T+C处理中被激发,对黄瓜促生贡献最大的分别归属于Parasediminibacterium、Fluviicola和Roseimaritima;有11个关键真菌OTU仅在T+C处理中被激发,对黄瓜促生贡献最大的分别归属于隔指孢属、Melanoleuca和毛壳菌属。[结论]相比于单一接种,丝竹虫和木霉组合接种后能更显著促进黄瓜生长,两者协同调控土著细菌和真菌群落,激发并富集潜在的土著功能微生物以塑造更有利于黄瓜生长的土壤微生物群落。[Objectives]The paper aimed to explore the effects of microbe-consuming protists,fungi and the microbe-consuming protists and growth-promoting fungi co-inoculation on cucumber growth and soil microbial community.[Methods]A greenhouse experiment was designed to decipher the role of keystone protists(Cercomomnas lenta)in interaction with plant-promoting Trichoderma guizhouense on the soil microbiome and cucumber performance.Four treatments were designed:no-inoculation control(CK),T.guizhouense inoculation treatment(T),Cercomomnas lenta inoculation treatment(C),and C.lenta and T.guizhouense co-inoculation treatment(T+C).Plants and soil samples were collected after two weeks of greenhouse experiment to evaluate the effects of each treatment on cucumber performance and soil bacterial and fungal communities.[Results]Compared to the control,plant height of T.guizhouense inoculation treatment,C.lenta inoculation treatment and C.lenta and T.guizhouense co-inoculation treatment increased by 6.0%,12.5%and 21.5%,respectively,while plant biomass increased by 5.6%,12.9%and 24.0%,respectively.This indicated that the growth promotion effect of C.lenta and T.guizhouense co-inoculation was stronger than those of individual inoculation of C.lenta and T.guizhouense.Principal coordinate analysis showed that three inoculation treatments significantly changed the bacterial and fungal community compositions;random forest models revealed that bacterial and fungal community compositions were the main predictors of plant biomass;and the LEfSe analysis showed that the T.guizhouense inoculation,C.lenta inoculation and C.lenta and T.guizhouense co-inoculation treatments stimulated 22,51 and 82 potential key bacterial OTU,and 2,6 and 16 potential key fungal OTU,respectively.In total 21 key bacterial and 11 key fungal OTU were only stimulated in the C.lenta and T.guizhouense co-inoculation treatment potentially contributing to cucumber growth.The bacterial OTU mostly contributing to cucumber growth promotion were assigned to Parasediminibacteri

关 键 词：黄瓜 原生动物 木霉 接种 土壤微生物群落 促生 

分 类 号：S154.37[农业科学—土壤学]