4种地黄红外光谱、热分析及微观结构对比研究  

Comparative Study on Infrared Spectrum,Thermal Analysis and Microstructure for Four Kinds of Radix rehmanniae

作　　者：章茂森[1] 丁融 吕年青 ZHANG Maosen;DING Rong;LYU Nianqing(Jiangsu Province Hospital of Traditional Chinese Medicine,Nanjing 210029,China;Jiangsu Health Development Research Center,Nanjing 210036,China)

机构地区：[1]江苏省中医院,江苏南京210029 [2]江苏省卫生健康发展研究中心,江苏南京210036

出　　处：《食品与生物技术学报》2023年第2期73-79,共7页Journal of Food Science and Biotechnology

基　　金：国家卫生健康委计划生育药具不良反应监测中心/江苏省卫生健康发展研究中心开放基金课题(JSHD2021007);江苏省卫生健康发展研究中心2021年度开放课题(JSHDRC2021007)。

摘　　要：为比较4种地黄(生地黄、熟地黄、生地炭、熟地炭)成分的相似性及差异性,采用傅立叶红外光谱仪测试了4种地黄红外光谱,并计算了它们的二阶导数图谱,用综合热分析仪分析了它们在受热过程中热性能变化,用X射线能谱仪和扫描电子显微镜分析了它们表面化学元素质量分数和微观结构。结果表明:4种地黄红外光谱主要吸收峰位置、峰形相似,但吸收峰强度有差异,它们均有地黄糖、醇和氨基酸等分子的—OH、—CH、C=O、—NH的吸收峰,其中熟地黄的糖质量分数较高,生地炭和熟地炭在多处吸收峰强度明显降低。在1200~500 cm^(-1)波段,4种地黄红外光谱二阶导数图在强度和形状均存在较大差异。在190~350℃温度段,由于地黄中多种糖类、醇、氨基酸等热分解导致较大的热失重,其中,生地黄和熟地黄比生地黄炭和熟地黄炭热失重更明显。生地炭和熟地炭比生地黄和熟地炭的氧元素质量分数低,而碳元素质量分数高,这些研究表明4种地黄化学成分相似,且生地黄和熟地黄有相似化学成分和微观结构,而生地炭和熟地炭有相似化学成分和微观结构,生地炭和熟地炭的糖、醇和氨基酸等质量分数比生地黄和熟地黄明显降低。In order to investigate and compare the similarities and differences of chemical composition of Rehmanniae radix,four-kind Rehmanniae radix(i.e.,Radix rehmanniae,Radix rehmanniae preparata,Radix rehmanniae charcoal,and Radix rehmanniae preparata charcoal,shorted for RR,RRP,RRC and RRPC,respectively)were studied by infrared spectroscopy(FTIR)and their second derivative spectra were calculated.Their thermal performance changes during heat treatment were analyzed with a comprehensive thermal analyzer,and their surface chemical element content and microstructure were analyzed by X-ray energy spectrometer and scanning electron microscope(SEM).The results showed that four kinds of Radix rehmanniae had similar absorption peak position and shape,however,their absorption peak intensity was different.They all had absorption peaks of-OH,-CH,C=O and-NH of polysaccharides,alcohols and amino acid groups of Radix rehmanniae,among which the content of polysaccharide was higher in the dry root of Radix rehmanniae,while the absorption strength of RRC and RRPC significantly reduced in many peaks.The intensity and shape of second derivative of infrared spectra of four-kind Radix rehmanniae were quite different at the 1200-500 cm-1 band.The four-kind Radix rehmanniae showed thermal weight loss due to the thermal decomposition of various polysaccharides,alcohols and amino acids under 190℃~350℃,and RR and RRP had significant thermal weight loss than that of RRC and RRPC.Compared with RR and RRP,RRC and RRPC had lower O element atomic content and higher C element atomic content were higher.These studies showed that the chemical composition was similar for four-kinds Radix rehmanniae,and RR and RRP had similar chemical composition and micro-structure,while RRC and RRPC had similar chemical composition and micro-structure.The content of polysaccharides and alcohols and amino acid in RRC and RRPC were significantly lower than that of RR and RRP.

关 键 词：地黄 红外光谱 热分析 微观结构 

分 类 号：R282.5[医药卫生—中药学]