黄荆子与蔓荆子特征图谱及酚类、黄酮类含量测定研究  

Study on characteristic chromatogram and content determination of phenols and flavonoids of Viticis Negundo Fructus and Viticis Fructus

作　　者：李国卫 何嘉莹 刘潇晗 邱韵静 钟春琳 吴文平 索彩仙 孙冬梅 LI Guo-wei;HE Jia-ying;LIU Xiao-han;QIU Yun-jing;ZHONG Chun-lin;WU Wen-ping;SUO Cai-xian;SUN Dong-mei(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司广东省中药配方颗粒企业重点实验室,佛山528244

出　　处：《天然产物研究与开发》2023年第3期379-386,396,共9页Natural Product Research and Development

基　　金：佛山市应急科技攻关专项(2020001000206)。

摘　　要：通过特征图谱及多指标含量测定对黄荆子和蔓荆子进行对比研究,在采用UPLC建立黄荆子药材特征图谱方法的基础上通过高分辨质谱对其化学成分进行指认,指认了其中9个成分,分别为原儿茶酸A、原儿茶醛、4-羟基苯甲酸、香草酸、异荭草苷、荭草苷、穗花牡荆苷、牡荆苷、异牡荆苷,并进行含量测定,15批黄荆子与12批蔓荆子的9个成分均有一定的差异,从多批次的均值来看,黄荆子的原儿茶酸、原儿茶醛、4-羟基苯甲酸、香草酸、穗花牡荆苷的含量高于蔓荆子,异荭草苷、荭草苷的含量低于蔓荆子;另外牡荆苷、异牡荆苷在蔓荆子中未有检出。本研究采用UPLC特征图谱结合多指标含量测定对黄荆子及蔓荆子进行对比研究,为黄荆子及蔓荆子的药材质量控制和临床用药提供参考。In order to study the differences between Viticis Negundo Fructus and Viticis Fructus,the characteristic chromatogram and multi-index content determination method was established.Based on the UPLC characteristic chromatogram of Viticis Negundo Fructus,nine chemical components were identified by high resolution mass spectrometry(HRMS)then determined,which were protocatechuic acid A,protocatechualdehyde,4-hydroxybenzoic acid,vanillic acid,isoorientin,orientin,agnuside,vitex and isovitex.The determination of 15 batches of Viticis Negundo Fructus and 12 batches of Viticis Fructus showed that there were differences among these nine components.The means of protocatechuic acid,protocatechualdehyde,4-hydroxybenzoic acid,vanillic acid and agnuside for Viticis Negundo Fructus were higher than Viticis Fructus that isoorientin and orientin were lower,while vitexin and isovitexin were not detected in Viticis Fructus.The UPLC characteristic chromatogram combined with multi-index content determination was applied to compare the differernces between Viticis Negundo Fructus and Viticis Fructus,which provide a reference for their quality control and clinical medication.

关 键 词：黄荆子 蔓荆子 高分辨质谱 特征图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]