细果角茴香内生真菌X-396的分离鉴定及其发酵物脂溶性成分分析  

Isolation and indentification of an endophytic fungus X-396 from Hypecoum leptocarpum Hook.f.et Thoms.and analysis on fat-soluble components of its fermentation

作　　者：谭慧 皮立[1] 杨小龙 安婷 郭旭栋 刘艳萍 文怀秀[1] TAN Hui;PI Li;YANG Xiao-long;AN Ting;GUO Xu-dong;LIU Yan-ping;WEN Huai-xiu(Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Research Institute of Forestry,Qinghai Academy of Agriculture and Forestry,Qinghai University,Xining 810016,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院西北高原生物研究所青海省藏药研究重点实验室,西宁810008 [2]青海大学农林科学院,西宁810016 [3]中国科学院大学,北京100049

出　　处：《天然产物研究与开发》2023年第3期420-426,共7页Natural Product Research and Development

基　　金：青海省科技厅项目(2021-SF-SW1);“青海省藏药研究重点实验室”(2022-ZJ-Y03)。

摘　　要：以藏药细果角茴香叶为材料,采用组织分离法分离纯化出一株内生真菌X-396。经形态学观察、ITS测序与序列比对鉴定内生真菌X-396为间座壳属(Diaporthe sp.)真菌Diaporthe sp.YG-2015。进一步利用气相色谱-质谱(GC-MS)联用技术对该真菌发酵物脂溶性成分进行检测与分析,并通过CCK-8法测定其抗肿瘤活性。结果表明,发酵物脂溶性成分共鉴定出35个,其中含量较高的成分为麦角甾醇(31.83%)、β-谷甾醇(12.00%)和十六酸甲酯(4.22%);体外抗肿瘤活性测试显示,发酵物脂溶性成分对人肿瘤细胞株A549、MCF-7和HepG2均具有不同程度的抑制作用,其中对HepG2抑制效果最好,IC 50为22.65μg/mL。本研究表明细果角茴香内生真菌X-396发酵物脂溶性成分中具有产生抗肿瘤活性化合物的潜力,具有进一步深入研究的价值。An endophytic fungus X-396 was isolated from the leaves of Hypecoum leptocarpum Hook.f.et Thoms.through a tissue separating method.The endophytic fungus X-396 was identified as Diaporthe sp.YG-2015 according to the morphologic observation and ITS sequence analysis.Its fat-soluble components of fermentation were further studied with GC-MS,and antitumor activity was measured by CCK-8 assay.35 kinds of fat-soluble components were detected and identified.The findings showed that the main components of fat-soluble components in fermentation of X-396 were ergosterol(31.83%),β-stitosterol(12.00%)and methyl hexadecanoate(4.22%).Antitumor activity test in vitro showed that the fat-soluble components of fermentation had different degrees of inhibitory effects on human tumor cell lines A549,MCF-7 and HepG2,and HepG2 had the best inhibitory effect with IC 50 of 22.65μg/mL.The results indicate that the fat-soluble components of endophytic fungus X-396 fermentation have the potential to produce antitumor active compounds,and deserve to be deeply investigated.

关 键 词：细果角茴香 内生真菌 发酵物 脂溶性成分 GC-MS 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学]