基于UHPLC-Q-Orbitrap-HRMS技术的司卡摩尼亚脂化学成分分析  

Chemical composition analysis of scammony resin by UHPLC-Q-Orbitrap-HRMS

作　　者：古丽米热·卡哈尔 热依木古丽·阿布都拉[1] 罗玉琴[1] 吴涛[1] 阿吉艾克拜尔·艾萨[1,2] KAHAR Gulmira;ABDULLA Rahima;LUO Yu-qin;WU Tao;AISA Hajiakber(Key Laboratory of Plant Resources and Chemistry in Arid Regions,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室,乌鲁木齐830011 [2]中国科学院大学,北京100049

出　　处：《天然产物研究与开发》2023年第3期427-443,共17页Natural Product Research and Development

基　　金：中国科学院仪器设备功能开发技术创新项目(2020 gz014);中国科学院王宽诚率先人才计划“产研人才扶持项目”;中国科学院青年创新促进会项目(2019)。

摘　　要：采用UHPLC-Q-Orbitrap-HRMS技术建立司卡摩尼亚脂中化学成分的快速分析方法。采用Full MS/dd-MS扫描模式采集质谱数据,根据获得的精确分子量、二级碎片信息,归纳总结裂解途径,结合文献报道和Compound Discover数据库预测,从司卡摩尼亚脂甲醇提取物中共鉴定出92个化合物,包括72个醚溶性树脂糖苷和树脂酸类成分、12个羟基肉桂酰基奎宁酸类和8个醚不溶性树脂酸类。其中,8个醚不溶性树脂酸类(化合物10、12、13、16、17、18、19、20)和2个三聚体奎宁酸类(化合物14、15)为本药材中首次发现的化合物。研究结果为今后司卡摩尼亚脂药材及其产品质量标准的建立提供科学依据。The rapid analysis method for the chemical compositions in scammony methanol extract was established by UHPLC-Q-Orbitrap HRMS.The mass data were acquired by Full MS/dd-MS scan mode,and data analysis were performed by the information of accuracy molecular weight,fragment ion,summarized fragmentation pathway,combined with previous references and Compound Discovery(CD)software.Totally,92 compounds were identified from scammony methanol extract,including 72 resin glycosides,12 hydroxyl cinnamoyl quinic acids,eight ether insoluble resin glycoside acids.Among them,eight ether insoluble resin glycoside acids(compounds 10,12,13,16,17,18,19 and 20)and two tricaffeoylquinic acids(compounds 14 and 15)were detected first in scammony.The results provide a scientific basis for the future research on the improvement of the quality standard for scammony and its relative products.

关 键 词：司卡摩尼亚脂 羟基肉桂酰基奎宁酸类 树脂糖苷类 UHPLC-Q-Orbitrap-HRMS 

分 类 号：R284.1[医药卫生—中药学]