基于响应面法结合总评归一法多指标优选蜜黄芪微波炮制工艺  

Optimization of microwave processing technology for honey roast Astragalus membranaceus by response surface method combined with overall desirability and normalization method with multi-index

作　　者：侯敏娜[1] 侯少平[1] 梁润丰 王珊[1] 许海燕[1] HOU Min-na;HOU Shao-ping;LIANG Rui-feng;WANG Shan;XU Hai-yan(Medical College,Shaanxi Institute of International Trade,Xianyang 712046,China)

机构地区：[1]陕西国际商贸学院,咸阳712046

出　　处：《天然产物研究与开发》2023年第3期467-476,共10页Natural Product Research and Development

基　　金：国家科技部重大新药创制科技重大专项(2018ZX 09721005-009-013);陕西省科技厅项目(2020SF-323);陕西国际商贸学院中药质量标志物创新团队(SSY18TD02);2022年陕西省教育厅重点科研计划(协同创新中心项目22JY005)。

摘　　要：基于响应面法结合总评归一法,优选蜜黄芪的微波炮制工艺。在单因素实验基础上,以蜜黄芪中总黄酮、总皂苷提取率、水溶性浸出物含量和饮片外观性状的总评归一值为评价指标,对蜂蜜用量、闷润时间、炮制时间和微波火力4个因素进行的响应面法优化实验研究。最终获得最佳工艺条件为:炮制时间81 s、蜂蜜用量34%、微波火力82%、闷润时间6.1 h,此时总黄酮、皂苷、水溶性浸出物含量分别为0.3510%、0.5894%、24.71%,饮片外观评分为90。本研究优选得到的微波蜜黄芪炮制工艺合理、稳定、可行,可为蜜黄芪的工业化生产提供理论参考依据,也为其他中药的炮制提供新的研究思路和研究方法。To optimize the microwave process of honey roast Astragalus membranaceus by response surface method(RSM)combined with overall desirability.On the basis of single factor experiment,the response surface optimization experiment was conducted on the four factors of honey roast A.membranaceus,honey dosage,stuffy moist time,processing time and microwave power by taking the total flavonoids,total saponin extraction rate,water-soluble extract content and the total evaluation normalization value of the appearance of the decoction pieces as evaluation indicators.Finally,the optimum technological conditions were obtained as follows:processing time of 81 s,honey dosage of 34%,microwave power of 82%,moist time of 6.1 h.At this time,the contents of total flavone,saponins,water-soluble extract were 0.3510%,0.5894%,24.71%and appearance score of slices was 90 respectively.The processing technology of microwave honey roast A.membranaceus was reasonable,stable and feasible,which can provide a theoretical reference for the industrial production of honey roast A.membranaceus,and also provide new research ideas and methods for the processing of other traditional Chinese medicines.

关 键 词：黄芪 响应面法 微波炮制 蜜炙 总评归一法 

分 类 号：R283[医药卫生—中药学]