宽体金线蛭不同工艺提取物的抗凝溶栓活性比较研究  

Comparison of Whitmania pigra Extracts From Different Processes Based on Anticoagulant and Thrombolytic Activity

作　　者：何盛盛 王水涛 陈姿亦 徐周熠 朱希忱 徐锦鹏 高有领[1] HE Shengsheng;WANG Shuitao;CHEN Ziyi;XU Zhouyi;ZHU Xichen;XU Jinpeng;GAO Youling(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo,Zhejiang 315100)

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《核农学报》2023年第1期88-97,共10页Journal of Nuclear Agricultural Sciences

基　　金：浙江省科技厅项目(LGN22C190028);宁波市科技局项目(202002N3048);浙江省生物工程一流学科学生创新计划项目(CX2020010)。

摘　　要：宽体金线蛭(Whitmania pigra)具有抗凝溶栓的药理作用,但其药效受提取工艺的影响。为全面评价并筛选出合适的提取工艺,本试验采用脱脂、水提、酸提和酶解等不同工艺制备了14种提取物,并对提取物的抗凝活性、纤溶活性与体外溶栓活性进行分析。结果表明,脱脂胃蛋白酶酶解提取物的抗凝血酶活性显著高于其他提取物,且具有较强的纤溶活性和体外溶栓活性;仿生酶解提取物具有较强的抗凝血酶活性和体外溶栓活性;脱脂仿生酶解提取物具有较强的纤溶活性和体外溶栓活性;脱脂加热的水提提取物具有较强的纤溶活性和体外溶栓活性。脱脂胃蛋白酶酶解提取物中,分子量小于3 kDa的多肽具有较强的抗凝血酶活性和纤溶活性。此外,三个水提提取物、仿生酶解物、脱脂仿生酶解物和粗酶酶解提取物对纤维蛋白原的α肽链均具有溶解作用。综上所述,宽体金线蛭提取物均具有抗凝溶栓的药理作用,酶解提取物优于水提提取物和酸提提取物,脱脂处理有利于活性物质的提取,其中胃蛋白酶酶解提取物效果最佳,并且脱脂胃蛋白酶酶解物的小分子多肽成分具有最佳的抗凝血酶活性和纤溶活性。本试验结果为进一步开发利用宽体金线蛭的药理作用提供了理论依据。Whitmania pigra is a traditional Chinese medicine with anticoagulation and thrombolysis.However,its pharmacological functions were affected by the extraction process.The aim of the present study was to evaluate the extracts from different processes,and finally screen the appropriate extraction process.The water extraction,acid extraction,degreasing and enzymatic hydrolysis was applied to prepare 14 extracts,in which the anticoagulant activity,fibrinolytic activity and in vitro thrombolytic activity were determined.The results showed that the degreasing pepsin hydrolysate had better parameters with antithrombin activity,fibrinolytic activity and in vitro thrombolytic activity.The biomimetic enzymatic hydrolysate had relatively high antithrombin activity and strong in vitro thrombolytic activity.Degreasing bionic enzyme hydrolysate had better fibrinolytic activity and in vitro thrombolytic activity.Degreasing heated water extraction extract had stronger fibrinolytic activity and in vitro thrombolytic activity.Degreasing pepsin hydrolysate played an anticoagulant fibrinolytic effect and the main components were the peptides with molecular weight less than 3 kDa.In addition,it was found that the three extracts from the water extraction,the extracts of biomimetic enzymatic hydrolysis,the degreasing biomimetic enzymatic hydrolysis and the crude enzymatic hydrolysis had a dissolving effect on theαpeptide chain of fibrinogen.In conclusion,the different extracts all had pharmacological effects of anticoagulant and thrombolytic.The enzymatic hydrolysis was better than water and acid extractions.Degreasing was beneficial to the extraction of the anticoagulant thrombolytic active substance.The pepsin hydrolysis was the best.The substance with low molecular weight had the best anticoagulant and fibrinolytic activities from the degreasing pepsin hydrolysate.The results of the present study set a theoretical basis for further developing pharmacological functions of Whitmania pigra.

关 键 词：宽体金线蛭 提取物 抗凝活性 纤溶活性 体外溶栓 

分 类 号：R285[医药卫生—中药学]