Natural variations of OsAUX5,a target gene of OsWRKY78,control the neutral essential amino acid content in rice grains  

作　　者：Yuheng Shi Yuanyuan Zhang Yangyang Sun Ziyang Xie Yu Luo Qiyuan Long Jiahui Feng Xueqing Liu Bi Wang Dujun He Junxia Ren Peizhen Guo Junwei Xing Liqiang He Alisdair RFernie Wei Chen Xianqing Liu Yuehua Luo Cheng Jin Jie Luo 

机构地区：[1]Sanya Nanfan Research Institute of Hainan University,Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China [2]College of Tropical Crops,Hainan University,Haikou 570228,China [3]Max Planck Institute of Molecular Plant Physiology,14476 Potsdam-Golm,Germany [4]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Molecular Plant》2023年第2期322-336,共15页分子植物（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(32170267 and 32001528);the Key Research and Development Program of Hainan(ZDYF2020066);the Hainan Province Science and Technology Special Fund(ZDYF2022XDNY261);the Hainan Major Science and Technology Project(ZDKJ202002 and ZDKJ202001);the Hainan Academician Innovation Platform(HD-YSZX-202003);the Hainan Yazhou Bay Seed Laboratory(B21Y10902).

摘　　要：Grain essential amino acid(EAA)levels contribute to rice nutritional quality.However,the molecular mechanisms underlying EAA accumulation and natural variation in rice grains remain unclear.Here we report the identification of a previously unrecognized auxin influx carrier subfamily gene,OsAUX5,which encodes an amino acid transporter that functions in uptake of multiple amino acids.We identified an elite haplotype of Pro::OsAUX5^(Hap2) that enhances grain EAA accumulation without an apparent negative effect on agronomic traits.Natural variations of OsAUX5 occur in the cis elements of its promoter,which are differentially activated because of the different binding affinity between OsWRKY78 and the W-box,contributing to grain EAA variation among rice varieties.The two distinct haplotypes were shown to have originated from different Oryza rufipogon progenitors,which contributed to the divergence between japonica and indica.Introduction of the indica-type Pro::OsAUX5^(Hap2) genotype into japonica could significantly increase EAA levels,indicating that indica-type Pro::OsAUX5^(Hap2) can be utilized to increase grain EAAs of japonica varieties.Collectively,our study uncovers an WRKY78–OsAUX5-based regulatory mechanism controlling grain EAA accumulation and provides a potential target for breeding EAA-rich rice.

关 键 词：amino acid natural variation transcriptional regulation Oryza sativa 

分 类 号：S511[农业科学—作物学]