健心颗粒对慢性心力衰竭气虚血瘀证模型大鼠心肌能量代谢及PPARα/PGC-1α通路的影响  

Effect of Jianxin Granules(健心颗粒)on Myocardial Energy Metabolism and PPARα/PGC-1αPathway in Model Rats of Chronic Heart Failure with Qi Deficiency and Blood Stasis Syndrome

作　　者：陈隽[1] 黄飞翔[1] 王永[1] 周一薇[1] 陈美华[1] 叶盈[1] CHEN Juan;HUANG Feixiang;WANG Yong;ZHOU Yiwei;CHEN Meihua;YE Ying(The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,350000)

机构地区：[1]福建中医药大学附属第二人民医院,福建省福州市350000

出　　处：《中医杂志》2023年第4期393-400,共8页Journal of Traditional Chinese Medicine

基　　金：福建省卫健委科技计划(2020GGA068);福建省自然科学基金(2022J01835)。

摘　　要：目的从心肌能量代谢角度探讨健心颗粒治疗慢性心力衰竭气虚血瘀证的可能作用机制。方法60只SD大鼠随机分为造模组50只和空白组10只。造模组大鼠采用冠脉结扎配合力竭式游泳、饥饿法建立慢性心力衰竭气虚血瘀证模型。造模成功46只大鼠随机分为模型组10只,缬沙坦组及健心颗粒低、中、高剂量组各9只。缬沙坦组给予7.2 mg/(kg·d)缬沙坦灌胃,健心颗粒低、中、高剂量组分别给予1.6、3.2、6.4g/(kg·d)剂量的健心颗粒灌胃,模型组和空白组每天仅给予1 ml/100 g无菌生理盐水灌胃。各组均每天灌胃2次,共4周。HE和Masson染色观察大鼠心肌组织病理改变。检测大鼠心肌组织能量代谢指标[包括糖原质量分数、脂肪酸(FA)及乳酸浓度、三磷酸腺苷(ATP)浓度]、左室射血分数(EF)、心肌组织过氧化物酶体增殖物激活受体α(PPARα)、过氧化物酶体增殖物激活受体γ辅助活化因子-1α(PGC-1α)、300 KDA腺病毒E1A结合蛋白(P300)、中链酰基辅酶A脱氢酶(MCAD)、腺苷酸转运体(ANT)蛋白及mRNA表达。结果HE和Masson染色结果显示,模型组大鼠心肌细胞重度肥大、排列松散,心肌纤维断裂,成纤维细胞严重增生并伴有炎细胞浸润,心肌纤维化严重,胶原组织增生明显,缬沙坦组和健心颗粒各剂量组心肌组织损伤和胶原组织增生均有不同程度改善。与空白组比较,其余各组大鼠心肌组织FA和乳酸浓度升高,EF、糖原质量分数、ATP浓度和PPARα、PGC-1α、P300、MCAD、ANT mRNA及蛋白表达降低(P<0.05);与模型组比较,缬沙坦组和健心颗粒各剂量组心肌组织FA和乳酸浓度降低,EF、糖原质量分数、ATP浓度和PPARα、PGC-1α、P300、MCAD、ANT mRNA及蛋白表达升高(P<0.05);且健心颗粒高剂量组各指标改善优于缬沙坦组和健心颗粒低、中剂量组(P<0.05),健心颗粒中剂量组各指标改善与缬沙坦组比较差异无统计学意义(P>0.05)。结论健心颗粒Objective To investigate the potential mechanism of Jianxin Granules(健心颗粒)in the treatment of chronic heart failure(CHF)with qi deficiency and blood stasis syndrome from the perspective of myocardial energy metabolism.Methods Sixty SD rats were randomly divided into modeling group(n=50)and blank group(n=10).The former established the rat model of CHF with qi deficiency and blood stasis syndrome by coronary artery ligation combined with exhaustive swimming and starvation.Forty-six rats were successfully modeled and were divided into model group(n=10),valsartan group(n=9)and Jianxin Granules low-,medium-and high-dose groups(n=9 each)according to the random number table.Valsartan group was given 7.2 mg/(kg·d)of valsartan by gavage,while the low-,medium-and high-dose groups of Jianxin Granules were given 1.6,3.2 and 6.4g/(kg·d)of Jianxin Granules by gavage,respectively.The model group and the blank group were given 1 ml/100 g of sterile normal saline by ga‐vage.The above interventions were administered twice a day for 4 weeks.Hematoxylin eosin(HE)and Masson stain‐ing were used to observe the pathological changes of rat myocardial tissue.The indexes of energy metabolism in rat myocardial tissues including glycogen mass fraction,concentration of fatty acid(FA)and lactic acid,and adenosine triphosphate(ATP)concentration were measured.Left ventricular ejection fraction(EF),peroxisome proliferator ac‐tivated receptorα(PPARα),peroxisome proliferator activated receptorγcoactivator-1α(PGC-1α),300kda adenovi‐rus E1A binding protein(P300),medium chain acyl CoA dehydrogenase(MCAD)and adenylate transporter(ANT)proteins and mRNA expressions were detected.Results HE and Masson staining showed that in the model group,cardiomyocytes were severely hypertrophic and loosely arranged,with broken myocardial fibers,seriously proliferated fibroblasts accompanied by inflammatory cell infiltration,as well as serious myocardial fibrosis and obvious collagen tissue hyperplasia.The myocardial tissues injury and collagen t

关 键 词：慢性心力衰竭 气虚血瘀证 心肌能量代谢 过氧化物酶体增殖物激活受体Α 过氧化物酶体增殖物激活受体γ辅助活化因子-1α 健心颗粒 

分 类 号：R285.5[医药卫生—中药学]