海沟泥样来源真菌Penicillium sp. HDN19010的次级代谢产物研究  

Secondary metabolites of Mariana Trench sediment-derived Penicillium sp. HDN19010

作　　者：刘峻妤 钱亚 王文雪 侯学文 朱天骄[1] 张国建[1,2] 车茜 李德海[1,2] LIU Jun-yu;QIAN Ya;WANG Wen-xue;HOU Xue-wen;ZHU Tian-jiao;ZHANG Guo-jian;CHE Qian;LI De-hai(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室医,药学院,山东青岛266003 [2]青岛海洋科学与技术国家实验室海洋药物和生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2023年第1期37-41,共5页Chinese Journal of Marine Drugs

基　　金：山东省自然科学基金重大基础研究项目(ZR2021ZD28)资助。

摘　　要：目的 研究马里亚纳海沟来源真菌Penicillium sp. HDN19010的次级代谢产物。方法 优化菌株培养条件,运用硅胶柱层析、ODS反相柱层析和半制备高效液相色谱法(HPLC)对其发酵产物进行分离提纯,通过质谱(MS)、核磁共振波谱(NMR)分析及和已报道文献比对,对化合物结构进行解析,并进行抑菌活性评价。结果 从该菌株中分离得到1个新的异戊烯基化的化合物methyl 2-[4-[(E)-4-hydroxy-3-methylbut-2-enoxy]phenyl]acetate(1),1个已知的二酮哌嗪类化合物fusaperazine F(2),2个已知二萜类化合物conidiogenone B~C(3~4)。评价了化合物1~4对10种细菌抑制活性评价,结果表明化合物均无抑菌活性。Objective To study the secondary metabolites of Mariana Trench sediment-derived Penicillium sp. HDN19010. Methods The culture conditions were optimized, and the strain extracts were isolated and purified by silica gel column chromatography and ODS reverse phase column chromatography combined with HPLC.The structures of compounds were elucidated by MS, NMR spectra, and by contrast with the dates of literatures. Then the antibacterial activities were evaluated. Results One new isopentenylated compound methyl 2-[4-[(E)-4-hydroxy-3-methylbut-2-enoxy]phenyl]acetate(1), one known diketopiperazine compound fusaperazine F(2) and two known diterpenoids conidiogenone B-C(3-4) were isolated from the fungus Penicillium sp. HDN19010. The antibacterial activities of compounds 1-4 against 10 kinds of bacteria were evaluated, and the results showed that none of the compounds had antibacterial activities.

关 键 词：深海 青霉 次级代谢产物 马里亚纳海沟 异戊烯基化 

分 类 号：R931[医药卫生—生药学]