放大镜目测法联合三磷酸腺苷生物荧光法在腹腔镜手术器械清洗效果检测中的应用分析  

Analysis of the visual inspection with magnifying lens combined with adenosine triphosphate bioluminescence assay in the cleaning effect detection of laparoscopic surgical instruments

作　　者：王继川 刘新 周树平 李娟 WANG Jichuan;LIU Xin;ZHOU Shuping;LI Juan(Department of Sterile Supply Room,the Reproductive Hospital of Guangxi Zhuang Autonomous Region,Nanning 530029,China)

机构地区：[1]广西壮族自治区生殖医院消毒供应室,广西南宁530029

出　　处：《机器人外科学杂志（中英文）》2023年第3期246-251,共6页Chinese Journal of Robotic Surgery

基　　金：广西壮族自治区卫生健康委员会自筹经费项目:AQP8在OHSS患者卵泡颗粒细胞中的表达及其对卵母细胞生长发育的影响(Z20190633)。

摘　　要：目的:观察放大镜目测法联合三磷酸腺苷(Adenosine Triphosphate,ATP)生物荧光法在腹腔镜手术器械清洗效果检测中的作用。方法:选择清洗后拟行低温等离子灭菌的腹腔镜手术器械72套,每套12件,随机分为三组,每组288件。A组采用放大镜目测法,B组采用裸视目测法联合ATP生物荧光法,C组采用放大镜目测法联合ATP生物荧光法,检测并记录各组器械的清洗不合格情况,记录B组和C组每套器械的检测费用。结果:检测出清洗不合格的器械分别为A组11件(3.8%)、B组23件(8.0%)、C组25件(8.7%),B组和C组检测出的器械清洗不合格率明显高于A组(P<0.05);C组每套器械的平均检测费用为(802.08±35.63)元,明显低于B组的(825.42±41.18)元(P<0.05)。结论:放大镜目测法联合三磷酸腺苷生物荧光法检测结果客观可靠,灵敏度高,检测费用低,适用于腹腔镜手术器械清洗效果的日常监测、重点加测或定期抽查。Objective:To observe the role of visual inspection with magnifying lens combined with adenosine triphosphate(ATP)bioluminescence assay in the detection of the cleaning effect of laparoscopic surgical instruments.Methods:72 sets of laparoscopic surgical instruments(12 pieces in each set)were selected to be sterilized by low-temperature plasma after cleaning and randomly divided into three groups,with 288 pieces in each group.visual inspection with magnifying lens was used in Group A,visual inspection combined with ATP bioluminescence assay were used in Group B,and visual inspection with magnifying lens combined with ATP bioluminescence assay was used in Group C to detect and record the cleaning failure of instruments in each group,and the detection cost of each set of instruments in Group B and Group C were also recorded.Results:11(3.8%),23(8.0%)and 25(8.7%)pieces of instruments in Group A,Group B and Group C respectively were detected as failing to clean,the failure rate of instrument cleaning detected in Group B and Group C was significantly higher than that in the Group A(P<0.05).The average testing cost per set of devices in Group C was(802.08±35.63)CNY,which was significantly lower than that in the Group B(825.42±41.18)CNY(P<0.05).Conclusion:The combination of visual inspection with magnifying lens and ATP bioluminescence assay is an objective and reliable method with high sensitivity and low cost,which is suitable for routine monitoring,additional testing or periodic spot checking the cleaning effect of laparoscopic surgical instruments.

关 键 词：放大镜目测法 三磷酸腺苷 生物荧光法 腹腔镜手术器械 器械清洗 

分 类 号：R197.39[医药卫生—卫生事业管理]