喜马拉雅旱獭心肌低氧适应的组织学特点  

Histological characteristics of myocardial hypoxic adaptation in Marmota himalayana

作　　者：李琳 秦鸿楠 周娟 万瑞东 申什菊 张勤文[1] LI Lin;QIN Hongnan;ZHOU Juan;WAN Ruidong;SHEN Shiju;ZHANG Qinwen(Department of Animal Medicine,College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Province Haidong Center for Animal Disease Control and Prevention,Haidong 814000,China)

机构地区：[1]青海大学农牧学院动物医学系,西宁810016 [2]青海省海东市动物疫病预防控制中心,青海海东814000

出　　处：《黑龙江畜牧兽医》2023年第5期127-131,140,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：青海省自然科学基金项目(2017-ZJ-718)。

摘　　要：为了阐明喜马拉雅旱獭心肌适应高原低氧环境的组织学特点,试验以5只成年喜马拉雅旱獭为研究对象,以SD大鼠为对照,采取左心室心肌组织,运用组织学技术研究其心肌肌纤维直径和表面积密度;免疫组织化学方法检测心肌组织血管内皮生长因子(VEGF)及微血管密度(MVD)等指标,运用电镜技术测算心肌线粒体的部分结构参数,包括平均体积、面数密度、体积密度等。结果表明:喜马拉雅旱獭心肌肌纤维直径比SD大鼠小,并存在显著差异(P<0.05),而喜马拉雅旱獭心肌肌纤维表面积密度大于SD大鼠且差异显著(P<0.05),喜马拉雅旱獭的VEGF含量和MVD均高于SD大鼠且差异显著(P<0.05),喜马拉雅旱獭心肌线粒体的平均截面积、平均体积均小于SD大鼠且差异显著(P<0.05),而喜马拉雅旱獭心肌线粒体的体积密度、面数密度均大于SD大鼠且差异显著(P<0.05)。说明与SD大鼠相比,喜马拉雅旱獭的心肌特点表现为肌纤维直径小、表面积密度大、VEGF含量和MVD高的特点,心肌线粒体表现为平均体积小、面数密度和体积密度大的特点,喜马拉雅旱獭心肌对高原具有低氧环境适应的组织学特征。In order to clarify the histological characteristics of the myocardium of Marmota himalayana adapted to the hypoxic environment at high altitude, in this study, five adult Marmota himalayana were selected as subjects, and SD rats were used as control. The myocardium tissue of left ventricle was used to study the myocardium muscle fiber diameter and surface area density. Immunohistochemical methods were used to measure vascular endothelial growth factor(VEGF) and microvascular density(MVD) in myocardial tissue, and electron microscopy was used to measure some structural parameters of Myocardial mitochondria, including average volume, surface density, volume density, etc.The myocardial muscle fiber diameter of marmota himalaya was smaller than that of SD rats, and the difference was significant(P<0.05), while the myocardial muscle fiber surface area density of Marmota himalaya was larger than that of SD rats, and the difference was significant(P<0.05).The VEGF content and MVD levels of Marmota himalaya were higher than those of SD rats and the difference was significant(P<0.05).The mean cross-sectional area and mean volume of myocardial mitochondria of Marmota himalaya were smaller than those of SD rats, and the differences were significant(P<0.05).The volume density and surface number density of myocardial mitochondria of Marmota himalaya were higher than those of SD rats, and the difference was significant(P<0.05).These results indicated that compared with SD rats, the myocardial characteristics of Marmota himalayana were smaller diameter of muscle fibers, larger surface area density, and higher content of VEGF and MVD. The myocardial mitochondria showed small mean volume, high surface density and high volume density. The myocardial muscle of Marmota himalaya had the histological characteristics of adaptation to low oxygen environment in high altitude.

关 键 词：喜马拉雅旱獭 心肌 低氧适应 组织学特点 线粒体 

分 类 号：S823.8[农业科学—畜牧学]