远志流浸膏中3种指标性成分的含量测定  

Determination of three index components in Polygala liquid extract

作　　者：陈涛 康闽 陈雪婷 林诗铃 林逸凡 郑燕芳[1] 黄鸣清[1] CHEN Tao;KANG Min;CHEN Xueting;LIN Shiling;LIN Yifan;ZHENG Yanfang;HUANG Mingqing(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《药学研究》2023年第2期105-108,共4页Journal of Pharmaceutical Research

基　　金：国家自然科学基金面上项目(No.81973437、82274080);福厦泉国家自主创新示范区协同创新平台专项(No.2021FX02)。

摘　　要：目的 建立测定远志流浸膏中3种指标性成分的含量的方法。方法 采用高效液相色谱(HPLC)法同时测定8批远志流浸膏中远志[口山]酮Ⅲ,3,6′-二芥子酰基蔗糖这两种成分含量,和高效液相色谱法测定碱水解远志流浸膏中细叶远志皂苷成分含量。结果 远志[口山]酮Ⅲ、3,6′-二芥子酰基蔗糖、细叶远志皂苷在各自测定范围内线性关系良好(r>0.999 9),平均加样回收率为98.76%~103.05%,RSD 0.82%~3.79%。结论 本试验方法结果准确、专属性好、重复性好、稳定性好,可为远志流浸膏的质量控制提供依据。Objective To establish a method for the determination of three index components in polygala liquid extract.Methods HPLC method was used to determine the content of polygalaxanthone III,3,6′-disinapoyl sucrose in 8 batches of Polygala liquid extract,and the content of tenuifolin in alkali-hydrolyzed polygala liquid extract was determined by HPLC.Results The linear relationships of polygalaxanthoneⅢ,3,6′-disinapoyl sucrose,and tenuifolin were good(r>0.9999).The average recoveries were 98.76%~103.05%with the RSD s of 0.82%~3.79%.Conclusion The results of this test method were accurate,specific,repeatable,and stable,which can provide a basis for the quality control of polygala liquid extract.

关 键 词：远志流浸膏 高效液相色谱 含量测定 远志[口山]酮Ⅲ 3 6′-二芥子酰基蔗糖 细叶远志皂苷 

分 类 号：R927.2[医药卫生—药学]