菌胁迫对近江牡蛎子代母源C型凝集素mRNA表达及抗菌影响  

On the Influence of Bacterial Stress on Maternal C-type Lectin m RNA Expression and Bacteriostasis in Crassostrea Rivularis Offspring

作　　者：王翠丽 WANG Cuili(Institute of Geography and Oceanography,Nanning Normal University,Nanning,Guangxi,530000,China;Key Laboratory of Environment Change and Resources Use in Beibu Gulf,,Nanning,Guangxi,530000,China)

机构地区：[1]南宁师范大学地理与海洋研究院,广西南宁530000 [2]南宁师范大学北部湾环境演变与资源利用教育部重点实验室,广西南宁530000

出　　处：《工业微生物》2023年第1期89-94,共6页Industrial Microbiology

基　　金：广西自然科学基金面上基金近江牡蛎C型凝集素母源免疫时效及传代机制研究2020GXNSFAA159088。

摘　　要：为了揭示近江牡蛎(Crassostrea rivularis)C型凝集素(C-type lectin,CTL)母源免疫在子代胚胎发育早期过程中的免疫防御影响,文章运用荧光定量PCR、人工促排、免疫相关酶活测定等实验,开展了菌胁迫近江牡蛎子代母源CTL m RNA表达及抗菌影响。结果表明:近江牡蛎子代胚胎发育早期可检测到母源CTL转录本,母贝分别在不同菌刺激后,其子代在受精卵、32细胞期、桑葚期、囊胚期CTL m RNA相对表达均出现不同程度的升高,与相应空白组比较差异显著(P<0.05);其子代在受精卵期、32细胞期的Cu/Zn-SOD活力和抑菌活性显著高于相应空白组(P<0.05);其子代在原肠胚期、担轮幼虫期累计死亡率均显著低于空白组(P<0.05)。结论:该近江牡蛎CTL具有母源免疫传代和子代免疫时效,当母源受到适当的免疫刺激可以增加其子代胚胎发育早期对相同病原感染的免疫防御能力。In order to reveal the effect of C-type lectin(CTL) maternal immunity during the early embryonic development of Crassostrea rivularis offspring, this paper was studied by using technologys of Real-time PCR, artificial ovulation promotion,determination of immune related enzyme activity and so on. Results showed that maternal CTL transcripts could be detected in the early embryonic development;when compared with the blank group, relative expressions of CTL mRNA were significantly increase at stage of fertilized egg, 32 cell, morula, blastula in offspring(P<0.05);Cu/Zn SOD activity and bacteriostatic activity were significantly increase at stage of fertilized egg, 32 cell in offspring(P<0.05);mortality rate were significantly lower at stage of gastrula, troehophore in offspring(P<0.05). Conclusion: this Crassostrea rivularis CTL has maternal immune passage and time effect in offspring;after the proper maternal bacterial challenge, it could be increase the immune defense ability at the offspring early embryonic development,when against the same bacterial infect.

关 键 词：菌胁迫 近江牡蛎 子代 C型凝集素 抗菌活力 

分 类 号：S917.4[农业科学—水产科学]