精氨酸对斑玉蕈菌丝生长的影响  

Effects of arginine on the mycelial growth of Hypsizygus marmoreus

作　　者：黄天宇 唐利华[2] 郑婷婷 万佳宁[2] 邹根 鲍大鹏[2] 李福后 龚明[2] HUANG Tianyu;TANG Lihua;ZHENG Tingting;WAN Jianing;ZOU Gen;BAO Dapeng;LI Fuhou;GONG Ming(College of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China;Southern Key Laboratory of Edible Fungus Resource Utilization,Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;College of Food Science&Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]江苏海洋大学食品科学与工程学院,江苏连云港222005 [2]上海市农业科学院食用菌研究所、农业农村部南方食用菌资源利用重点实验室、国家食用菌工程技术研究中心、国家食用菌加工技术研发分中心、上海市农业遗传育种重点开放实验室,上海201403 [3]上海海洋大学食品学院,上海201306

出　　处：《菌物学报》2023年第2期609-619,共11页Mycosystema

基　　金：国家自然科学基金(32072642);“科技创新行动计划”“一带一路”国际合作项目(20310741900)。

摘　　要：采用平板培养和基因表达等方法,研究了精氨酸对斑玉蕈菌丝生长的影响及其机理。结果表明添加1–2 mmol/L精氨酸能明显提高菌丝的生长速度。添加精氨酸促进精氨酸代谢,提高GCN2介导的翻译通路活力以及空泡氨基酸转运载体基因AVT3的表达。营养补充实验证实在营养匮乏的情况下,单一添加精氨酸能促进斑玉蕈菌丝良好生长。研究结果证实外源添加精氨酸能作为主要营养物质促进斑玉蕈菌丝生长。The effects of arginine on the mycelial growth of Hypsizygus marmoreus were studied by plate culture and gene expression.The results showed that the addition of 1–2 mmol/L arginine to the medium could obviously increase the growth rate of mycelium.Additional arginine stimulated arginine metabolism and elevated the activity of the GCN2-mediated translation pathway and expression of the vacuolar amino acid transporter gene AVT3.Nutrient supplement experiments confirmed that pure addition of arginine could promote mycelial growth of Hypsizygus marmoreus under nutrient deprivation.

关 键 词：斑玉蕈 精氨酸 菌丝生长 GCN2 翻译通路 

分 类 号：S64[农业科学—蔬菜学]