γ干扰素体外释放试验在人和动物结核病诊断中的研究进展  

Research Progresses on Interferon-γ Release Assay for Diagnosis ofHuman and Animal Tuberculosis

作　　者：刘蒙达 王晓文 张皓博 亓菲 沈叶盛 苏华彬 张研博 张力[5] 李岩[5] 范伟兴[1] 孙淑芳[1] 樊晓旭[1] LIU Meng-da;WANG Xiao-wen;ZHANG Hao-bo;QI Fei;SHEN Ye-sheng;SU Hua-bin;ZHANG Yan-bo;ZHANG Li;LI Yan;FAN Wei-xing;SUN Shu-fang;FAN Xiao-xu(National Animal Tuberculosis Reference Laboratory,Division of Zoonoses Surveillance,China Animal Health and Epidemiology Center,Qingdao 266032,China;Chinese Society For Measurement,Beijing 100125,China;College of Animal Technology,Shandong Agricultural University,Taian 271000,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;General Station of Animal Husbandry and Veterinary,The Xinjiang Production and Construction Corps,Tacheng 832104,China)

机构地区：[1]中国动物卫生与流行病学中心人兽共患病监测室国家动物结核病参考实验室,山东青岛266032 [2]中国计量测试学会,北京朝阳100125 [3]山东农业大学动物科技学院,山东泰安271000 [4]青岛农业大学动物医学院,山东青岛266109 [5]新疆生产建设兵团畜牧兽医工作总站,新疆塔城832104

出　　处：《中国兽医杂志》2023年第3期91-97,共7页Chinese Journal of Veterinary Medicine

基　　金：现代农业(奶牛)产业技术体系建设专项(CARS-36);新疆生产建设兵团财政科技计划项目(2020AB015)。

摘　　要：结核病是给人类和动物健康造成严重影响的主要人兽共患传染病。早发现、早诊断、早干预对于有效地预防和控制结核病流行至关重要。γ干扰素是一种参与宿主抗结核感染的细胞免疫反应的细胞因子,基于其开发的γ干扰素体外释放试验可用于结核病诊断。鉴于目前酶联免疫吸附试验检测γ干扰素的方法存在诸多缺陷,例如耗时长、灵敏度低、无法区分潜伏性和活动性结核感染等,生物传感器正被尝试用于γ干扰素检测,以进一步提高诊断的灵敏性和特异性。本文归纳了将γ干扰素作为结核病生物标志物的多种检测技术,并讨论其适用范围和效果,以期为完善结核病诊断技术及开发新型诊断方法提供理论和技术参考。Tuberculosis is a major zoonotic infectious disease with serious consequences on human and animal health.Early detection,diagnosis and intervention are crucial for effectively prevention and control of this epidemic disease.As a cytokine involved in the host's cellular immune response against tuberculosis infection,interferon-γhas been exploited in interferon-γrelease assay for the diagnosis of tuberculosis.However,given the obvious shortcomings of enzyme-linked immunosorbent assay(ELISA)-based detection of interferon-γ,including time-consuming feature,low sensitivity,and inability to distinguish latent infection from active tuberculosis,biosensors are increasingly applied to further improve its sensitivity and specificity.By presenting an overview on tuberculosis detection techniques using interferon-γas a biomarker and discussions on their efficiency and utility,this article hopes to provide theoretical and practical sources for the future refinement of tuberculosis diagnosis methodologies.

关 键 词：结核病 牛结核病 Γ干扰素释放试验 生物传感器 

分 类 号：S855.2[农业科学—临床兽医学]