槐花散对细菌内毒素诱导的肠上皮细胞炎性损伤的保护作用及其机制  

Protective Effect and Mechanism of Huaihua San on Endotoxin-InducedInflammatory Injury in Intestinal Epithelial Cells

作　　者：吕喜凯 戴文 刘萍 刘钟杰[2] LYU Xi-kai;DAI Wen;LIU Ping;LIU Zhong-jie(College of Animal Science and Technology&Veterinary Medicine,Zhejiang A&F University,Hangzhou 310000,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]浙江农林大学动物科技学院·动物医学院,浙江杭州310000 [2]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2023年第3期136-139,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(31972727);浙江农林大学科研发展基金项目(2021FR002)。

摘　　要：为了探究槐花散对肠上皮细胞炎性损伤的保护作用及其机制,本试验用细胞内毒素脂多糖(LPS)诱导肠上皮细胞系IEC-6损伤模型,首先通过CCK8法筛选槐花散水提液的作用浓度,继而通过蛋白免疫印迹法检测NOD样受体蛋白3(NLRP3)炎性小体主要成分NLRP3、ASC和caspase-1及其下游白细胞介素-18(IL-18)蛋白的表达水平,综合评价槐花散对NLRP3炎性小体介导的肠上皮细胞炎性损伤的作用。结果显示,不同浓度的槐花散皆能显著抑制LPS诱导的IEC-6细胞炎性小体激活,并降低下游IL-18炎性因子表达(P<0.05)。结果表明,槐花散可能通过抑制炎性小体激活以保护肠上皮细胞抵抗LPS诱导的细胞炎性损伤。This study investigated the protective effect and the mechanism of Huaihua San on intestinal epithelial cell inflammatory injury through application of a model of intestinal epithelial cell line IEC-6 injury induced by lipopolysaccharide(LPS).Firstly,the active concentrations of Huaihua San aqueous extract were screened by CCK8 assay,and the protein expression levels of NLRP3,ASC and caspase-1,the main components of NLRP3 inflammasome,as well as the downstream interleukin-18(IL-18)were then detected by Western blot assay.The results showed that Huaihua San of different concentrations significantly inhibited LPS-induced NLRP3 inflammasome activation and the downstream IL-18 expression in IEC-6 cells(P<0.05).It thus appears that Huaihua San protects intestinal epithelial cells against LPS-induced cell inflammatory injury through inhibition of NLRP3 inflammasome activation.

关 键 词：槐花散 NLRP3炎性小体 肠炎 炎性损伤 

分 类 号：S853.74[农业科学—临床兽医学]