枣CML基因的分子特征及其抗寒性表达模式分析  

Molecular characteristics of CML genes in Chinese jujube and their expression patterns in resistance to cold stress

作　　者：高梦娇 王林霞 罗智 赵若雨 刘志国 刘平[1,2] 刘孟军[1,2] 王立新[1,2] Gao Mengjiao;Wang Linxia;Luo Zhi;Zhao Ruoyu;Liu Zhiguo;Liu Ping;Liu Mengjun;Wang Lixin(College of Horticulture,Hebei Agricultural University,Baoding 071001,Hebei,China;Research Center of Chinese Jujube,Hebei Agricultural University,Baoding 071001,Hebei,China)

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北农业大学中国枣研究中心,河北保定071001

出　　处：《北京林业大学学报》2023年第3期58-67,共10页Journal of Beijing Forestry University

基　　金：国家自然科学基金青年科学基金项目(32201594);河北省教育厅科学技术研究项目(QN2022017);河北省省属高校基本科研业务费研究项目(KY2021059);河北省引进留学人员资助项目(C20210114);国家重点研发计划课题(2019YFD1001605)。

摘　　要：【目的】探究枣类钙调蛋白(ZjCML)在抗寒性中的重要作用,旨在挖掘关键抗寒ZjCML基因,为进一步利用其进行枣抗寒育种提供理论参考。【方法】本研究利用生物信息学技术,全面分析了枣CML基因家族成员的数目及相关结构特征,并利用RNA-seq和实时荧光定量PCR技术分析其响应低温胁迫时的表达模式,为筛选关键抗寒基因奠定基础。【结果】在枣基因组数据中共鉴定到23个ZjCML基因,不均匀地分布在12条染色体上。与拟南芥CMLs蛋白构建进化树分析发现ZjCMLs可被分为12个类群。蛋白网络互作预测发现16个ZjCMLs存在于该网络中,且具有一定的互作关系。RNA-seq结果表明ZjCML13和ZjCML6基因表达模式在‘冬枣’及其同源四倍体响应低温胁迫中具有显著差异,其中ZjCML13在‘冬枣’响应低温胁迫6和24 h时极显著上调表达,而在‘冬枣’同源四倍体中表达差异不显著。外源Ca Cl2和褪黑素处理后,ZjCML13在‘冬枣’同源四倍体响应低温胁迫6和24 h时极显著上调表达,说明其可能通过诱导ZjCML13表达调控‘冬枣’同源四倍体抗寒性。【结论】枣CML基因家族具有特定的结构特征及响应低温胁迫,ZjCML13可能在调控‘冬枣’及其同源四倍体抗寒性差异中发挥重要作用。[Objective] This paper aims to investigate the important founction of CaM-like proteins in Chinese jujube in resistance to cold stress and identify the key ZjCML,which could provide theoretical basis for cold breeding in Chinese jujube by using them.[Method] Through using bioinformatics technology,the number and related structural information of CML in Chinese jujube were analyzed comprehensively in the current study.And then,we used RNA-seq and real-time fluorescence quantitative PCR technology to analyze its expression pattern in response to low temperature stress,laying a foundation for screening key cold resistance genes.[Result] A total of 23 ZjCML genes were identified in Chinese jujube genome.All of them distributed unevenly on 12 chromosomes.In addition,ZjCML can be divided into 12 subgroups,and protein interaction network prediction showed that 16 ZjCML existed in the network and they had mutual interaction relationships.Moreover,RNA-seq analysis showed that the expression patterns of ZjCML13 and ZjCML6 in response to cold stress between ‘Dongzao' and its autotetraploids were significantly different.The expression level of ZjCML13 in response to cold stress in ‘Dongzao' was significantly higher than that in its autotetraploid at 6 and 24 h.External CaCl2 and melatonin treatments could significantly induce the expression of ZjCML13 in autotetraploids ‘Dongzao' under cold stress at 6 and 24 h,indicating that it may regulate cold resistance of autotetraploid ‘Dongzao' by inducing the expression of ZjCML13.[Conclusion] The ZjCML gene family have specific characteristics and expression patterns,ZjCML13 might play important role in regulation of cold difference between ‘Dongzao' and its autotetraploids.

关 键 词：枣 类钙调蛋白(CML) 基因家族 抗寒性 表达分析 

分 类 号：S722.36[农业科学—林木遗传育种] S665.1[农业科学—林学]