亚洲百合部分品种灰霉病抗性评价及抗病生理响应  

Evaluation of resistance to Botrytis elliptica and the physiological response of some Asian lily varieties

作　　者：胡浩 汪莲娟 隗功磊 陈佳伟 贾桂霞[1] Hu Hao;Wang Lianjuan;Wei Gonglei;Chen Jiawei;Jia Guixia(Beijing Key Laboratory of Ornamental Plant Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Beijing Green Garden Group,Beijing 100067,China)

机构地区：[1]花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,林木花卉遗传育种教育部重点实验室,北京林业大学园林学院,北京100083 [2]北京花乡花木集团有限公司,北京100067

出　　处：《北京林业大学学报》2023年第3期104-112,共9页Journal of Beijing Forestry University

基　　金：重要花卉种质资源精准评价与基因发掘(2019YFD1000400);河北省重点研发计划项目-花卉现代种业科技创新团队(21326317D)。

摘　　要：【目的】为筛选出抗灰霉病能力强的亚洲百合品种,对部分Tiger、Pearl系列亚洲百合品种及兰州百合进行灰霉病抗性评价并初步探究其抗病的生理机制。【方法】对不同品种亚洲百合进行离体叶片接菌,评价其相对抗病能力,并测定筛选出的抗病和感病品种接菌后的生理变化。【结果】(1)Tiger、Pearl系列百合品种及兰州百合对灰霉病的抗性存在差异。其中抗灰霉病能力相对较强的品种是‘Pearl Melanie’,属于中抗水平;抗性最弱的是‘Pearl Justin’、兰州百合、‘Pearl White’和‘White Twinkle’。(2)供试百合的抗性水平与其气孔密度、气孔大小无显著相关性。(3)相对抗病的品种‘Pearl Melanie’和感病品种‘Pearl Justin’在接菌后的生理响应显著不同。随着接菌时间的延长,丙二醛的含量总体呈增加趋势,草酸氧化酶和几丁质酶的活性逐渐增强,β-1,3-葡聚糖酶的活性随时间的延长呈下降趋势。其中抗病品种的丙二醛含量相对低于感病品种,草酸氧化酶和几丁质酶的活性高于感病品种。【结论】叶片气孔的性状与抗病能力无显著相关性,抗病能力不同的百合在生理机制上有一定差异。在生理响应过程中,丙二醛含量、草酸氧化酶活性和几丁质酶活性可作为筛选亚洲百合对灰霉病抗性的评价指标。[Objective] This paper aims to select Asiatic lilies with strong resistance to Botrytis elliptica,the resistance of some Tiger and Pearl series Asian lilies and Lilium davidii var.unicolor to B.elliptica was evaluated,and the physiological mechanism of resistance was preliminarily explored.[Method] The disease resistance of different cultivars or species was evaluated by ex vivo leaf inoculation.On this basis,the physiological changes of selected disease-resistant and susceptible cultivars after inoculation were determined.[Result] The comprehensive evaluation results showed that:(1) the resistance of Tiger,Pearl lilies and L.davidii var.unicolor to B.elliptica was different.Relatively strong resistance to B.elliptica was‘Pearl Melanie',which belonged to the medium resistance level.The weakest resistance materials were‘Pearl Justin',L.davidii var.unicolor,‘Pearl White' and ‘White Twinkle'.(2) There was no significant correlation between resistance level and stomatal density,stomatal size.(3) The physiological responses of the resistant cultivar ‘Pearl Melanie' and susceptible cultivar ‘Pearl Justin' were obviously different after inoculation.The content of MDA showed an increasing trend with the extension of inoculation time,while the activities of oxalate oxidase and chitinase gradually increased,and the activities of β-1,3-glucanase decreased with time.The MDA content of the resistant cultivars was relatively lower than susceptible cultivar,and the activities of oxalate oxidase and chitinase were higher than susceptible cultivar.[Conclusion] There is no significant correlation between stomatal characteristics and disease resistance,the physiological mechanism of lillies with distinct disease resistance is different.During the physiological response,MDA content,oxalate oxidase activity and chitinase activity can be used as evaluation indexes for evaluating Asiatic lilies for resistance to B.elliptica.

关 键 词：亚洲百合 灰霉菌 抗性评价 生理响应 

分 类 号：S682.2[农业科学—观赏园艺]