自还原MXene负载金的适体传感器检测小米样品中的黄曲霉毒素B_(1)  

Detection of Aflatoxin B_(1)by Self-reducing Au NPs@MXene Aptasensor in Millet Samples

作　　者：杨梦 李紫滢 陈志雄 廖文椿 陈本奇 黄龙 孟爽 杨云慧[1] 胡蓉[1] 杨通 YANG Meng;LI Zi-ying;CHEN Zhi-xiong;LIAO Wen-chun;CHEN Ben-qi;HUANG Long;MENG Shuang;YANG Yun-hui;HU Rong;YANG Tong(College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,Yunnan,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《食品研究与开发》2023年第6期163-170,共8页Food Research and Development

基　　金：国家自然科学基金项目(21765026)。

摘　　要：该文研究一种基于负载金纳米颗粒(Au nanopaticles,Au NPs)的MXene复合纳米材料(Au NPs@MXene)的电化学适体传感器,可对黄曲霉毒素B_(1)(aflatoxin B_(1),AFB_(1))进行敏感检测。利用了MXene特殊的二维结构及其自身的还原性,简单、快速、高效地将Au NPs原位生长在其表面。所合成的材料具有优异的导电性、比表面积较大、较电催化活性好,可作为电化学生物传感器的基底材料。在最佳试验条件下,传感器对AFB_(1)的检出限为6.3 pg/mL(信噪比为3),线性范围为0.05 ng/mL~100 ng/mL。该传感器已应用于小米样品中AFB_(1)的测定,且在其他食品基质中也具有较大潜力。In this paper,an electrochemical aptasensor based on MXene composite nanomaterials loaded with gold nanoparticles(Au NPs@MXene)was studied,which could sensitively detect aflatoxin B_(1)(AFB_(1)).Using the special two-dimensional structure of MXene and its reducibility,Au NPs could be grown in situ on its surface simply,quickly,and efficiently.The synthesized material had excellent conductivity,large specific surface area,and better electrocatalytic activity,which could be used as the substrate material of electrochemical biosensor.Under the optimum experimental conditions,the detection limit of AFB_(1)by the reported sensor was 6.3 pg/mL(S/N=3),and the linear range was 0.05 ng/mL-100 ng/mL.It has been applied to the determination of AFB_(1)in millet samples and has great potential in other food substrates.

关 键 词：金纳米颗粒 MXene 自还原 电化学 适体传感器 黄曲霉毒素B_(1) 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] TP212[轻工技术与工程—食品科学与工程]