金钱松中二萜类化学成分及其体外抗肿瘤活性研究  

Diterpenoid constituents in Pseudolarix amabilis and their antitumor activities in vitro

作　　者：王尚谊 朱裕勋 张召欣 杨程硕 夏慧敏 苏国柱 李勇[1] WANG Shang-yi;ZHU Yu-xun;ZHANG Zhao-xin;YANG Cheng-shuo;XIA Hui-min;SU Guo-zhu;LI Yong(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院、北京协和医学院药物研究所、天然产物生物活性物质和功能国家重点实验室,北京100050

出　　处：《中国中药杂志》2023年第1期96-104,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(21977119,22107120)。

摘　　要：运用多种色谱方法和波谱方法从金钱松Pseudolarix amabilis种子95%乙醇冷浸提取物的二氯甲烷部位分离鉴定了17个松香烷型二萜类化合物,分别为pseudoamaol A (1)、12α-hydroxyabietic acid (2)、12-methoxy-7,13-abietadien-18-oic acid (3)、13-hydroxy-8,11,13-podocarpatrien-18-oic acid (4)、15-hydroxy-7,13-abietadien-12-on-18-oic acid (5)、8(14)-podocarpen-13-on-18-oic acid (6)、holophyllin K (7)、metaglyptin B (8)、7α-hydroxydehydroabietinsaure-methylester (9)、7-oxodehydroabietic acid (10)、15-hydroxy-7-oxodehydroabietinsaure-methylester (11)、15-methoxydidehydroabietic acid (12)、7-oxo-15-hydroxy-dehydroabietic acid (13)、15-hydroxydehydroabietic acid (14)、8,11,13-abietatriene-15,18-diol (15)、8,11,13-abietatriene-15-hydroxy-18-succinic acid (16)、7β-hydroxydehydroabietic acid (17),其中化合物1为新化合物。对分离得到的化合物进行了3种肿瘤细胞株(HepG2、SH-SY5Y、K562)的体外抗肿瘤活性筛选,结果表明化合物8和17对K562细胞具有一定的细胞毒活性,其IC50分别为26.77、37.35μmol·L^(-1)。By various chromatographic techniques and extensive spectroscopic methods, 17 abietane diterpenoids were isolated from the dichloromethane fraction of the 95% ethanol cold-soak extracts of the seeds of Pseudolarix amabilis, namely pseudoamaol A(1), 12α-hydroxyabietic acid(2), 12-methoxy-7,13-abietadien-18-oic acid(3), 13-hydroxy-8,11,13-podocarpatrien-18-oic acid(4), 15-hydroxy-7,13-abietadien-12-on-18-oic acid(5), 8(14)-podocarpen-13-on-18-oic acid(6), holophyllin K(7), metaglyptin B(8), 7α-hydroxydehydroabietinsaure-methylester(9), 7-oxodehydroabietic acid(10), 15-hydroxy-7-oxodehydroabietinsaure-methy-lester(11), 15-methoxydidehydroabietic acid(12), 7-oxo-15-hydroxy-dehydroabietic acid(13), 15-hydroxydehydroabietic acid(14), 8,11,13-abietatriene-15,18-diol(15), 8,11,13-abietatriene-15-hydroxy-18-succinic acid(16), and 7β-hydroxydehydroabie-tic acid(17). Compound 1 was a new compound. The isolated compounds were evaluated for their antitumor activities(HepG2, SH-SY5Y, K562), and compounds 8 and 17 showed potential cytotoxic activity against K562 cells, with IC50values of 26.77 and 37.35 μmol·L^(-1), respectively.

关 键 词：金钱松 二萜 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]