自然衰老大鼠的肠道菌群演变  

Evolution of intestinal flora in naturally aging rats

作　　者：姜河 刘珊[2] 常超[3] 耿婕 Jiang He;Liu Shan;Chang Chao;Geng Jie(Department of Cardiology,the Affiliated Chest Hospital of Tianjin University,Tianjin 300051,China;Institute of Cardiology,the Affiliated Chest Hospital of Tianjin University,Tianjin 300222,China;Cardiac Surgery Intensive Care Unit,the Affiliated Chest Hospital of Tianjin University,Tianjin 300051,China)

机构地区：[1]天津大学附属天津市胸科医院心血管内科,天津300051 [2]天津大学附属天津市胸科医院心脏病研究所,天津300222 [3]天津大学附属天津市胸科医院心脏外科重症监护室,天津300051

出　　处：《中国医学前沿杂志（电子版）》2023年第3期46-52,共7页Chinese Journal of the Frontiers of Medical Science(Electronic Version)

基　　金：天津市卫生健康科技项目(ZC20126);天津市医院协会医院管理研究项目(2019ZC13);天津市医学重点学科(专科)建设项目(TJYXZDXK-055B)。

摘　　要：目的探究大鼠在自然衰老过程中肠道菌群组成的演变过程。方法在无特定致病菌环境中饲养8月龄雄性无特定病原体级的SD大鼠5只,分别于19、20、21、22、23、24月龄时取大鼠粪便进行16s核糖体RNA检测以分析肠道菌群的丰度及组成。结果大鼠肠道菌群的丰度及多样性随月龄的增加而降低。19~21月龄大鼠肠道菌群的组成相似,以厚壁菌门和拟杆菌门为主,且占比接近,22~24月龄大鼠厚壁菌门相对丰度显著减少,而拟杆菌门相对丰度显著增多。不同月龄大鼠肠道菌群的差别主要存在于厚壁菌门、拟杆菌门、放线菌门和变形菌门等,月龄越大的老年大鼠肠道菌群中益生菌丰度越低,致病菌丰度越高。结论自然衰老大鼠随着月龄增加肠道菌群的丰度和多样性也随之发生演变,为老年大鼠及人群的肠道菌群及疾病研究提供了数据参考。Objective To explore the evolution of intestinal flora in rats during natural aging.Methods Five 8-month-old male rats were raised without specific pathogenic bacteria.At the age of 19,20,21,22,23 and 24 months,the abundance and diversity of their intestinal flora were detected by 16s ribosomal RNA(16s rRNA).Results The abundance and diversity of intestinal flora in rats decreased with increasing age.The composition of intestinal flora of 19-21 month-old rats was similar,with predominating Firmicutes and Bacteroides,equally.The relative abundance of Firmicutes in 22-24 month-old rats decreased,while Bacteroides increased significantly.The differences of intestinal flora of rats at different months of age were mainly in Firmicutes,Bacteroides,Actinobacteria and Proteobacteria.Older rats had lower abundance of probiotic bacteria and higher abundance of pathogenic bacteria in the intestinal flora with aging.Conclusions The abundance and diversity of intestinal flora of rats change during the natural aging,providing data reference for the research of intestinal flora and diseases in elderly rats and people.

关 键 词：衰老大鼠 肠道菌群 微生物 16s rRNA 高通量测序 

分 类 号：Q95-33[生物学—动物学]