2型糖尿病阴虚证病证结合动物模型的建立与评价  

Establishment and Evaluation of Animal Model of Type 2 Diabetes with Yin-Deficiency Syndrome

作　　者：孙晓霞 任威铭 陈宁[1] 崔荣兴 翁泽斌[1] 战丽彬[1] SUN Xiao-xia;REN Wei-ming;CHEN Ning;CUI Rong-xing;WENG Ze-bin;ZHAN Li-bin(School of Traditional Chinese Medicine&School of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing,210023)

机构地区：[1]南京中医药大学中医学院·中西医结合学院,南京210023 [2]辽宁中医药大学中医药创新工程技术中心,沈阳110847

出　　处：《中国中西医结合杂志》2023年第2期200-211,共12页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点研发计划资助项目(No.2018YFC1704400);国家中医药领军人才岐黄学者支持项目(No.国中医药人教函[2022]6号);江苏省研究生科研创新计划(No.KYCX21_1700)。

摘　　要：目的建立2型糖尿病阴虚证病证结合动物模型,制定模型评价方法。方法SPF级雄性db/db小鼠随机分为糖尿病组(简称D组)10只和病证结合模型组(简称M组)40只,同窝m/m小鼠10只为正常组(简称C组)。第1~4周为阴虚证造模阶段:M组每日灌服伤阴药,余组灌服等体积超纯水。阴虚证造模结束后,将M组随机分为糖尿病阴虚证组(简称DY组)、六味地黄汤低、中、高剂量组(分别简称为DY-L、DY-M、DY-H组),每组10只。第5~8周为六味地黄汤干预阶段:DY-L、DY-M、DY-H组每日增加灌服相应剂量六味地黄汤(0.1 mL/10 g),余组灌服等体积超纯水。每周检测小鼠空腹血糖、体温、水量及唾液流率、皮肤含水量、粪便含水量、24 h尿量、体质量、食量和拉力;第8周末进行口服葡萄糖耐量实验(OGTT)、胰岛素耐量实验(ITT)及旷场实验。结果阴虚证造模4周后,与C组比较,D组与M组空腹血糖升高,D组OGTT及ITT实验曲线下面积(AUC)增高(P<0.05,P<0.01);与D组比较,M组小鼠四肢爪心温度、心前区温度及肛温、水量增加,唾液流率、四肢及背部皮肤含水量、粪便含水量、24 h尿量、体重、食量、拉力降低(P<0.05,P<0.01);六味地黄汤干预4周后,与D、DY组比较,给药各组小鼠旷场区运动总距离和速度、中央格穿格次数、四肢爪心温度、心前区温度及肛温、水量下降,唾液流率、背部和右下肢皮肤含水量、粪便含水量、24 h尿量、体重、拉力增加(P<0.05,P<0.01)。结论自发性2型糖尿病模型结合耗伤阴液法可成功建立2型糖尿病阴虚证病证结合动物模型。动态检测宏观表征及客观指标,结合以方测证是2型糖尿病阴虚证病证结合模型系统性评价的一种重要方法。Objective To establish an animal model of type 2 diabetes combined with Yin-deficiency syndrome and develop a model evaluation method. Methods SPF male db/db mice were randomly divided into the diabetes group(10 mice,group D)and the combined disease and syndrome model group(40 mice,group M). Ten littermate m/m mice were in the control group(group C). The model-building stage of Yin-deficiency syndrome was week 1-4. Mice in group M were intragastrically administered with Yin-damaging drugs daily,and the remaining groups were intragastrically administered with an equal volume of ultrapure water. After the modeling of Yin-deficiency syndrome,group M was randomly divided into diabetes Yin-deficiency syndrome group(group DY),Liuwei Dihuang Decoction low,medium,and high dose groups(group DY-L,DY-M,DY-H) with 10 mice per group. The intervention phase of Liuwei Dihuang Decoction was Week 5-8. The DY-L,DY-M,and DY-H groups were given corresponding doses of Liuwei Dihuang Decoction daily(0.1 mL/10 g),and the remaining groups were given the same volume of ultrapure water. Weekly detection of fasting blood glucose,temperature and rectal temperature,water volume and saliva flow rate,skin water content,fecal water content,24-hour urine volume,body weight,food volume,and tension in mice. At the end of the 8th week,oral glucose tolerance test(OGTT),insulin tolerance test(ITT) and open field test were performed. Results After 4 weeks of Yin-deficiency syndrome modeling,compared with group C,fasting blood glucose increased in group D and group M,and the AUC of OGTT and ITT increased in group D(P<0.05, P<0.01). Compared with group D,the temperature of claws centers,precordial temperature,rectal temperature and water volume of mice in group M increased,while the saliva fow rate,the skin water content of limbs and back,fecal water content,24 h urine volume,body weight,food intake and pulling force decreased(P<0.05,P<0.01). After 4 weeks of intervention with Liuwei Dihuang Decoction,compared with group D and group DY,total distance an

关 键 词：2型糖尿病 阴虚证 病证结合动物模型 中医 

分 类 号：R259[医药卫生—中西医结合] R-332[医药卫生—中医内科学]