硫熏对百合药材质量和安全的影响  

Effect of sulfur fumigation on quality and safety of Lilii Bulbus

作　　者：张蓉 杨亚玲 邓爱平 康利平[2] 程铭恩 康传志 郭兰萍[2,4] ZHANG Rong;YANG Ya-ling;DENG Ai-ping;KANG Li-ping;CHENG Ming-en;KANG Chuan-zhi;GUO Lan-ping(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;State Key Laboratory Base of Daodi Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Hubei University of Chinese Medicine,Wuhan 430065,China;Key Laboratory of Biology and Cultivation of Herb Medicine,Ministry of Agriculture and Rural Affairs,Bejing 100700,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中国中医科学院、中药资源中心、道地药材国家重点实验室培育基地,北京100700 [3]湖北中医药大学,湖北武汉430065 [4]农业农村部中药材生物学与栽培重点实验室,北京100700

出　　处：《中国中药杂志》2023年第3期660-671,共12页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(82104389);中央级公益性科研院所基本科研业务费专项(ZZXT202108);中国中医科学院科技创新工程项目(CI2021A03905)。

摘　　要：百合作为药食两用常用中药,市场硫熏严重,而硫熏对百合药材质量和安全的影响值得关注。该研究采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS/MS)结合主成分分析(PCA)及正交偏最小二乘判别分析(OPLS-DA)等多元统计分析方法对百合硫熏前后的差异成分进行分析,解析了硫熏后新生成的10个含硫标志物,对其进行了质谱裂解规律及转化规律总结,并对其中的苯丙烯酸类含硫标志物进行了结构验证。同时,对百合硫熏前后的水提液进行了细胞毒性评价,结果表明,0~800 mg·L^(-1),硫熏百合水提液对人肝LO2细胞、人肾近曲小管细胞HK-2和大鼠肾上腺嗜铬细胞瘤细胞PC-12的细胞活力无明显影响,与硫熏前的水提液无显著差异。该研究首次鉴定了硫熏百合中苯丙烯酸类以及呋甾皂苷类成分的含硫标志物,并从安全性角度明确了百合适当硫熏不会产生细胞毒性,为硫熏百合的快速鉴别和质量安全控制提供理论依据。Lilii Bulbus is a commonly used Chinese herbal medicine with both medicinal and edible values, while the market products usually has the problem of sulfur fumigation. Therefore, the quality and safety of Lilii Bulbus products deserve attention. In this study, ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was combined with principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) to analyze the differential components of Lilii Bulbus before and after sulfur fumigation. We identified ten markers generated after sulfur fumigation, summarized their mass fragmentation and transformation patterns, and verified the structures of phenylacrylic acid markers of sulfur fumigation. At the same time, the cytotoxicity of the aqueous extracts of Lilii Bulbus before and after sulfur fumigation was evaluated. The results showed that in the concentration range of 0-800 mg·L^(-1), the aqueous extract of Lilii Bulbus after sulfur fumigation had no significant effect on the viability of human liver LO2 cells, human renal proximal tubular HK-2 cells, and rat adrenal pheochromocytoma PC-12 cells. Moreover, the viability of the cells exposed to the aqueous extract of Lilii Bulbus before and after sulfur fumigation showed no significant difference. This study identified phenylacrylic acid and furostanol saponins as markers of sulfur-fumigated Lilii Bulbus for the first time, and made clear that proper sulfur fumigation of Lilii Bulbus would not produce cytotoxicity, providing a theoretical basis for the rapid identification and quality and safety control of sulfur-fumigated Lilii Bulbus.

关 键 词：百合 硫熏 UPLC-Q-TOF-MS/MS 含硫标志物 细胞毒性 

分 类 号：R284.1[医药卫生—中药学]