甘肃农田两种土壤纤毛虫形态学和细胞发生学  

Morphology and morphogenesis of two farmland soil ciliates from Gansu

作　　者：杨怀印 王春慧 韩海峰 王丽[1] 陈凌云[1] 宁应之[1] YANG Huai-yin;WANG Chun-hui;HAN Hai-feng;WANG Li;CHEN Ling-yun;NING Ying-zhi(School of Life Science,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《兰州大学学报（自然科学版）》2023年第1期36-46,共11页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(41761056,42067012);甘肃省自然科学基金项目(20JR5RA532);环境化学与生态毒理学国家重点实验室开放基金项目(KF2021-02)。

摘　　要：利用非淹没培养皿法、活体显微观察法和蛋白银染色法,对采自甘肃农田的2种土壤纤毛虫,即中华异拟片尾虫和殖口后殖口虫进行形态学和细胞发生学研究,揭示殖口后殖口虫横棘毛的来源.结果表明,中华异拟片尾虫在活体状态下呈长椭圆形,细胞质无色,具有深绿色皮层颗粒,两枚椭圆形大核,其细胞发生学主要特征:老口围带完整保留,被前仔虫所继承;6列额-腹-横棘毛原基以典型的1∶3∶3∶3∶4∶4模式分化棘毛;左、右缘棘毛原基分别由老结构反分化形成;背触毛发生为“拟片尾虫”模式.殖口后殖口虫在活体状态下呈披针型,两端稍尖,在虫体中部达到最宽,皮层颗粒缺失,其细胞发生学主要特征:老口围带完整保留,被前仔虫所继承;6列额-腹-横棘毛原基以1∶2∶2∶2∶4∶n模式形成棘毛;左、右缘棘毛原基缘分别由老结构反分化形成;背触毛原基发生为“殖口虫”模式.The morphology and morphogenesis of two new records of farmland soil ciliates,Heterourosomoida sinica and Metagonostomum gonostomoida,collected from Gansu were investigated using the nonflooded Petri dish method,live observation and protargol staining.The results indicated that the outline of H.sinica was elliptical in vivo,cytoplasm colorless,of dark green cortical granules and with two elliptical macronuclei.The main morphogenetic features were described as follows:the parental adoral zone was inherited intactly by the proter;the frontal-ventral-transverse cirral anlagen-Ⅵwere generated in the following numbers of cirri 1∶3∶3∶3∶4∶4;marginal cirri anlagen were generated within the parental marginal rows;development of dorsal kineties followed the Urosomoida-pattern.The outline of M.gonostomoida was lanceolate in vivo,slightly pointed at both ends and widest at the middle of the body,with cortical granules being absent.The main morphogenetic features were described as follows:the parental adoral zone was inherited intactly by the proter;the frontal-ventral-transverse cirral anlagen were generated in the following numbers of cirri 1∶2∶2∶2∶4∶n;marginal cirri anlagen were generated within the parental marginal rows;development of dorsal kineties followed the Gonostomum-pattern.

关 键 词：农田 新纪录纤毛虫 形态学 细胞发生学 

分 类 号：Q959[生物学—动物学]