利用CLIA法筛查重庆口岸出入境人员梅毒抗体  

CLIA method was used to screen syphilis antibody among international travelers in Chongqing port

作　　者：倪丹妮 蒋颖 周鑫 周冠邑 何传宇 郝玉通 NI Danni;JIANG Ying;ZHOU Xin;ZHOU Guanyi;HE Chuanyu;HAO Yutong(Chongqing International Travel Healthcare Center(Chongqing Customs Port Outpatient Department),Chongqing 401147,China)

机构地区：[1]重庆国际旅行卫生保健中心(重庆海关口岸门诊部),重庆401147

出　　处：《中国国境卫生检疫杂志》2023年第1期65-66,共2页Chinese Journal of Frontier Health and Quarantine

基　　金：重庆海关科技计划项目(2021CQKJ07)。

摘　　要：目的 探讨应用全自动化学发光免疫分析仪检测出入境人员梅毒抗体,确定化学发光法(CLIA)检测的最佳S/CO临界值。方法 选取2018年8月—2022年1月的142份经CLIA法初筛梅毒抗体阳性(S/CO≥1)的样本,用CLIA法复检,两次检验结果一致的标本再用TPPA确认,绘制ROC曲线获取最佳临界值。结果 CLIA复检阳性标本140份,TPPA检测阳性100份,两种方法阳性符合率为71.43%。根据ROC曲线,得到CLIA法检测出入境人员梅毒抗体S/CO最佳临界值为2.685,此时CLIA法和TPPA检测的符合率为95.6%。结论 基于CLIA法获得出入境人员梅毒抗体的S/CO最佳临界值,可指导实验室减少TPPA法的确诊试验,为提高检测时效提供参考。Objective To investigate the application of automatic chemiluminescence immunoassay(CLIA) to detect syphilis antibody of entry-exit personnel,and determine the optimal S/CO threshold value of CLIA. Methods From August 2018 to January 2022,142 syphilis antibody positive samples(S/CO≥1) initially screened by CLIA method were selected and re-examined by CLIA method. The samples with the same results were confirmed by TPPA. ROC curve was drawn to obtain the optimal critical value. Results A total of 140 samples were positive re-examined by CLIA,100 samples were positive in TPPA,the positive coincidence rate of two methods was 71.43%. According to ROC curve,the optimal critical value of CLIA for the detection of syphilis antibody S/CO among entry and exit personnel was 2.685,and the coincidence rate of CLIA and TPPA was 95.6%. Conclusion CLIA method was used to obtain the optimal S/CO threshold value of syphilis antibody of entry-exit personnel,which can guide the laboratory to reduce the TPPA method of diagnostic tests,and provide reference for improving the detection efficiency.

关 键 词：化学发光免疫分析法 梅毒螺旋体颗粒凝集试验 梅毒抗体 

分 类 号：R183.5[医药卫生—流行病学]