基于碱提多糖收率及含量的茯苓质量评价方法研究  

Quality evaluation methods of Poria based on yield and content of alkali extracted polysaccharide

作　　者：王悦 刘晓谦[1] 张永欣[1] 冯伟红[1] 闫利华[1] 王智民[1] WANG Yue;LIU Xiao-qian;ZHANG Yong-xin;FENG Wei-hong;YAN Li-hua;WANG Zhi-min(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]天津中医药大学,天津301617

出　　处：《中国中药杂志》2023年第2期443-454,共12页China Journal of Chinese Materia Medica

基　　金：中国中医科学院科技创新工程项目(CI2021A04407,CI2021A04406);国家药典委员会国家药品标准提高项目(2019Z019,2018Z005);国家重点研发计划“中医药现代化研究”重点专项(2017YFC1701900);国家中药标准化项目(ZYBZH-C-JL-24)。

摘　　要：为了完善茯苓的质量控制方法,更好地开发利用茯苓资源,该文采用碱液浸提法提取茯苓碱溶性浸出物,通过单因素分析,确定了碱溶性浸出物的最佳提取方法,并对30批样品进行测定。采用高效凝胶色谱(HPGPC)、红外光谱(FT-IR)、核磁共振光谱(NMR)、1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱(PMP-HPLC)等方法分析碱溶性浸出物的化学组成。结果表明,30批茯苓样品碱溶性浸出物在46.98%~73.86%;茯苓碱溶性浸出物的主要成分为β-(1→3)-葡聚糖,相对分子质量约为1.093×10^(5)。进一步,分别采用紫外-可见分光光度法(UV-Vis)和高效液相色谱-蒸发光散射检测法(HPLC-ELSD),建立了茯苓碱溶性多糖的含量测定方法,并测定30批样品。结果表明,UV-Vis测定茯苓碱溶性多糖在73.70%~92.57%,不同产地样品含量为湖北略高于云南、安徽、湖南。HPLC-ELSD测定茯苓碱溶性多糖为51.42%~76.69%,不同产地样品含量为湖南略高于湖北、安徽、云南。可见,UV-Vis测定的碱溶性多糖含量偏高;HPLC-ELSD的测定结果与碱溶性浸出物的含量较为接近,能够较准确地表征茯苓碱提多糖的含量,且方法简便,重复性好。因此,建议将碱溶性浸出物和碱溶性多糖HPLC-ELSD定量分析方法纳入茯苓质量标准控制项目,作为其《中国药典》标准的补充。To improve the quality control methods of Poria and develop and utilize its resources fully,alkaline extraction was used in this study to determine the yield and content of alkali-soluble polysaccharides of Poria.The alkali-soluble extracts of Poria were obtained according to the optimum extraction conditions on the basis of single-factor test,and 30 batches of samples were determined.The structure and chemical composition of the alkali-soluble extracts was characterized by high-performance gel permeation chromatography(HPGPC),Fourier transform infrared spectrometry(FT-IR),nuclear magnetic resonance(NMR)spectroscopy and high-performance liquid chromatography(HPLC)with 1-phenyl-3-methyl-5-pyrazolone(PMP-HPLC).The results showed that the content of the alkali-soluble extracts was in the range of 46.98%-73.86%.The main component wasβ-(1→3)-glucan,and its molecular mass was about 1.093×10^(5).Further,the content of alkali-soluble polysaccharides of Poria was measured by UV-Vis spectrophotometry and HPLC coupled with the evaporative light scattering detector(HPLC-ELSD),and 30 batches of samples were measured.The results indicated that the content of alkali-soluble polysaccharides determined by UV-Vis spectrophotometry was in the range of 73.70%-92.57%,and the content of samples from Hubei province was slightly higher than that from Yunnan province,Anhui province and Hunan province.The content of alkali-soluble polysaccharides determined by HPLC-ELSD was in the range of 51.42%-76.69%,and the samples from Hunan province had slightly higher content than that from the other three provinces.The content determined by UV-Vis spectrophotometry was higher than that by HPLC-ELSD.However,the content determined by HPLC-ELSD was close to that of alkali-soluble extract,which could accurately characterize the content of alkali-soluble polysaccharides in Poria,and the method was simple and repeatable.Therefore,it is recommended that the quantitative analysis method for alkali-soluble extract and alkali-soluble polysaccharides by

关 键 词：茯苓 碱溶性浸出物 碱溶性多糖 含量测定 1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱法 高效液相色谱-蒸发光散射检测法 

分 类 号：R284.1[医药卫生—中药学]