祛痰化瘀中药复方对心房颤动大鼠心房肌超微结构及氧化应激的影响  

Effects of TCM compounds for Eliminating phlegm and removing blood stasis on the ultrastructure of atrial myocardium and the oxidative stress in rats with atrial fibrillation

作　　者：吴启华 宫丽鸿[2] 康渊强 高明宇 Wu Qihua;Gong Lihong;Kang Yuanqiang;Gao Mingyu(Doctoral Degree Student of Grade 2019,School of Integrative Chinese and Western Medicine,Liaoning University of Traditional Chinese Medicine,Shenyang 110033,China;Department of Geriatrics and Geriatrics,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;Medical Department of Jinqiu Hospital,Liaoning Province,Shenyang 110067,China;Health Commission of Liaoning Province,Shenyang 110001,China)

机构地区：[1]辽宁中医药大学中西医结合学院2019级博士研究生,沈阳110033 [2]辽宁中医药大学附属医院老年病干诊科,沈阳110032 [3]辽宁省金秋医院医务科,沈阳110067 [4]辽宁省卫生健康委员会,沈阳110001

出　　处：《国际中医中药杂志》2023年第3期303-307,共5页International Journal of Traditional Chinese Medicine

基　　金：辽宁省特聘教授课题项目(辽教函〔2018〕35号)。

摘　　要：目的观察祛痰化瘀中药复方对心房颤动大鼠心房肌超微结构的影响,探讨其作用机制。方法将60只雄性SD大鼠按随机数字表法分为空白组10只和造模组50只,造模组连续7 d尾静脉注射氯化乙酰胆碱(ACh)-无水氯化钙(CaCl2)混合液建立房颤模型,将造模成功大鼠按随机数字表法分为模型组、维拉帕米组及中药高、中、低剂量组,每组10只。中药高、中、低剂量组分别灌胃祛痰化瘀中药复方汤剂41.25、20.63、10.31 g/(kg•d),维拉帕米组灌胃盐酸维拉帕米片溶液25 g/(kg•d),空白组及模型组灌胃等体积生理盐水,连续灌胃14 d。采用电生理记录仪测量各组大鼠房颤持续时间;透射电镜观察各组大鼠心房肌细胞超微结构;ELISA法检测各组大鼠血清活性氧自由基(ROS)、SOD、GSH-Px水平。结果与模型组比较,中药高、中、低剂量组及维拉帕米组大鼠房颤持续时间缩短(P<0.05);心房肌细胞超微结构受损改善;中药高、中剂量组及维拉帕米组血清ROS[(139.20±3.34)ng/ml、(139.00±3.28)ng/ml、(139.25±3.82)ng/ml比(155.60±7.32)ng/ml]水平降低,SOD[(2.41±0.26)ng/ml、(2.40±0.12)ng/ml、(2.37±0.06)ng/ml比(2.12±0.21)ng/ml]水平升高(P<0.05);中药高、中、低剂量组及维拉帕米组GSH-Px[(3.61±0.06)ng/ml、(3.60±0.08)ng/ml、(3.47±0.15)ng/ml、(3.51±0.19)ng/ml比(3.27±0.12)ng/ml]水平升高(P<0.05)。结论祛痰化瘀中药复方可缩短大鼠房颤持续时间,减轻房颤大鼠心房肌细胞超微结构的损伤,调节房颤大鼠血清ROS、SOD、GSH-Px水平,抑制氧化应激反应,这可能是其治疗房颤的作用机制之一。Objective To observe the effects of TCM compounds for eliminating phlegm and removing blood stasis on the ultrastructure of atrial myocardium in atrial fibrillation(AF)rats;To explore its possible mechanism.Methods Totally 60 male SD rats were divided into blank group(10 rats)and model group(50 rats)according to random number table method.The atrial fibrillation model was established by tail vein injection of ACh-CaCl2 mixture for 7 consecutive days,and then the rats with successful modeling were divided into model group,verapamil group,TCM compounds high-,medium-and low-dosage groups according to random number table method,with 10 rats in each group.TCM compounds high-,medium-and low-dosage groups were given Chinese herbal decoction 41.25,20.63,10.31 g/(kg•d)by gavage,respectively,and the verapamil group was given verapamil solution 25 g/(kg•d).The blank group and the model group were given an equal volume of normal saline by gavage for consecutive 14 days.After 14 consecutive days,electrophysiological recorder was used to measure the duration of atrial fibrillation in each group of rats;the changes of ultrastructure of atrial myocytes in each group were observed under transmission electron microscope;serum ROS,SOD and GSH-Px levels of rats were detected by ELISA.Results Compared with the model group,the duration of atrial fibrillation in TCM compounds high-,medium-and low-dosage groups and verapamil group decreased(P<0.05);the ultrastructural damage of atrial myocytes was improved;the content of ROS[(139.20±3.34)ng/ml,(139.00±3.28)ng/ml,(139.25±3.82)ng/ml vs.(155.60±7.32)ng/ml]in TCM compounds high-and medium-dosage groups and verapamil group decreased;the contents of SOD[(2.41±0.26)ng/ml,(2.40±0.12)ng/ml,(2.37±0.06)ng/ml vs.(2.12±0.21)ng/ml]increased(P<0.05);the content of GSH-Px[(3.61±0.06)ng/ml,(3.60±0.08)ng/ml,(3.47±0.15)ng/ml,(3.51±0.19)ng/ml vs.(3.27±0.12)ng/ml]in TCM compounds high-,medium-and low-dosage groups and verapamil group increased(P<0.05).Conclusion TCM compounds for eliminatin

关 键 词：心房颤动 氧化性应激 祛痰化瘀 复方(中药) 大鼠 

分 类 号：R285.5[医药卫生—中药学]