黄芪多糖对小鼠S180肉瘤的抑制作用及其机制研究  

Inhibitory effect of astragalus polysaccharide on S180 sarcoma in mice and its mechanism

作　　者：张炜 华翔 李竹莲 ZHANG Wei;HUA Xiang;Li Zhulian(Department of Pharmacy,Affiliated Hospital of Jinggangshan University,Ji′an,Jiangxi 343000,China;Department of Pharmacy,LePing People′s Hospital,Jingdezhen,Jiangxi 333300,China;Department of Pharmacy,Xingan People′s Hospital,Ji′an,Jiangxi 331300,China)

机构地区：[1]井冈山大学附属医院药剂科,江西吉安343000 [2]乐平市人民医院药剂科,江西景德镇333300 [3]新干县人民医院药剂科,江西吉安331300

出　　处：《现代医药卫生》2023年第6期917-920,923,共5页Journal of Modern Medicine & Health

基　　金：江西省中医药管理局科技项目(2021B128)。

摘　　要：目的探讨黄芪多糖(APS)对小鼠S180肉瘤的抑制作用及其机制。方法选取50只小鼠,建立小鼠S180肉瘤小鼠模型,将小鼠随机分为对照组、卡铂组、APS高剂量组(100 mg/kg)、APS中剂量组(80 mg/kg)、APS低剂量组(40 mg/kg),每组10只。比较各组肉瘤重量、肉瘤体积、抑瘤率、脾指数、胸腺指数,以及肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)、白细胞介素(IL)-2、IL-6水平。结果APS高剂量组、APS中剂量组、卡铂组肉瘤重量、体积小于对照组,差异有统计学意义(P<0.01)。APS高剂量组肉瘤重量、体积与APS低剂量组比较,差异有统计学意义(P<0.01)。APS高剂量组、APS中剂量组、APS低剂量组、卡铂组抑瘤率分别为50.32%、48.22%、35.69%、73.52%,差异有统计学意义(P<0.05)。卡铂组脾、胸腺指数低于对照组,差异有统计学意义(P<0.01)。不同APS剂量组脾、胸腺指数与对照组比较,差异无统计学意义(P>0.05)。APS高剂量组、APS中剂量组TNF-α、IFN-γ、IL-2、IL-6水平与对照组比较,差异有统计学意义(P<0.05)。APS高剂量组IL-2、IL-6水平与APS低剂量组比较,差异有统计学意义(P<0.05)。结论APS具有抗S180肉瘤活性,且不损害免疫功能,其抗肿瘤机制可能与调控细胞因子水平有关。Objective To investigate the inhibitory effect of astragalus polysaccharide(APS)on S180 sarcoma in mice and its mechanism.Methods 50 mice were selected to establish the S180 sarcoma mouse model.The mice were randomly divided into the control group,the carboplatin group,the APS high-dose group(100 mg/kg),APS middle-dose group(80 mg/kg)and the APS low-dose group(40 mg/kg),with 10 mice in each group.The sarcoma weight,sarcoma volume,inhibition rate,spleen index,thymus index and the levels of tumor necrosis factorα(TNF-α),interferonγ(IFN-γ),interleukin(IL)-2 and IL-6 in each group were compared.Results The weight and volume of sarcoma in the APS high-dose group,the APS middle-dose group and the carboplatin group were smaller than those in the control group,and the differences were statistically significant(P<0.01).The weight and volume of sarcoma in the APS high-dose group were significantly different from those in the APS low-dose group(P<0.01).The inhibition rates of the APS high-dose group,the APS middle-dose group,the APS low-dose group and the carboplatin group were 50.32%,48.22%,35.69%and 73.52%respectively,with significant differences(P<0.05).The indexes of spleen and thymus in the carboplatin group were lower than those in the control group,and the differences were statistically significant(P<0.01).There were no significant differences in the spleen and thymus indexes among the different APS dose groups and the control group(P>0.05).The levels of TNF-α,IFN-γ,IL-2 and IL-6 in the APS high-dose group and the APS middle-dose group were significantly different from those in the control group(P<0.05).The levels of IL-2 and IL-6 in the APS high-dose group were significantly different from those in the APS low-dose group(P<0.05).Conclusion APS has anti-S180 sarcoma activity and does not damage immune function.Its anti-tumor mechanism may be related to the regulation of cytokines level.

关 键 词：黄芪多糖 细胞因子 免疫功能 

分 类 号：R967[医药卫生—药理学]