慢性阻塞性肺疾病患者血清IL-19、IL-21的表达及其与免疫功能的关系  

Expression of serum interleukin-19 and interleukin-21 in patients with chronic obstructive pulmonary disease and their relationship with immune function

作　　者：晏园君 邓丽 YAN Yuanjun;DENG Li(Department of Laboratory,the Third Affiliated Hospital of Southern Medical University,Guangzhou,510600,China;Institute of Pulmonary Disease,Guangzhou Chest Hospital)

机构地区：[1]南方医科大学第三附属医院检验科,广州510600 [2]广州市胸科医院肺部疾病研究所

出　　处：《临床血液学杂志》2023年第2期104-108,112,共6页Journal of Clinical Hematology

摘　　要：目的:观察慢性阻塞性肺疾病(COPD)患者血清白细胞介素-19(IL-19)、白细胞介素-21(IL-21)水平,并分析两者与患者免疫功能的关系。方法:选择2020年4月至2022年1月收治的120例COPD患者为研究对象,所有患者均参照《慢性阻塞性肺疾病诊治指南(2013版)》给予常规治疗,采集患者入院时、入院7 d、14 d空腹静脉血,检测患者IL-19、IL-21及免疫功能指标T淋巴细胞亚群CD3+、CD4+、CD8+,分析COPD患者血清IL-19、IL-21与免疫功能的关系。结果:入院7 d、14 d时,患者IL-19、IL-21、CD8+水平较入院时逐步降低,CD3+、CD4+与入院时比较逐步升高,差异有统计学意义(P<0.05);经广义估计方程分析,以入院时作为参照,以IL-19作为协变量,COPD患者入院7 d时CD3+、CD4+分别升高4.790%、14.106%,14 d时CD3+、CD4+分别升高13.573%、19.964%,CD8+降低5.345%(P<0.05);以IL-21作为协变量,COPD患者入院7 d时CD3+、CD4+分别升高6.942%、21.275%,14 d时CD3+、CD4+分别升高15.058%、23.738%,CD8+降低2.633%(P<0.05)。COPD患者CD3+、CD4+、CD8+T淋巴细胞水平与IL-19、IL21水平有关(P<0.05)。结论:COPD患者血清IL-19、IL-21水平随着治疗时间延长而逐渐降低,且二者表达水平与CD3+、CD4+呈负相关,与CD8+呈正相关。Objective: To observe the levels of serum interleukin-19(IL-19) and interleukin-21(IL-21) in patients with chronic obstructive pulmonary disease(COPD), and analyze the relationship between them and immune function. Methods: A total of 120 COPD patients admitted from April 2020 to January 2022 were selected as the research subjects. All patients were given routine treatment according to the Guidelines for the Diagnosis and Treatment of Chronic Obstructive Pulmonary Disease(2013 Edition). Fasting venous blood was collected at admission, 7 d and 14 d after admission, and IL-19, IL-21 and immune function indexes(T lymphocyte subsets CD3+, CD4+, CD8+)were detected. The relationship between serum IL-19, IL-21 and immune function in patients with COPD was analyzed. Results: Seven day and 14 day after admission, the levels of IL-19, IL-21 and CD8+were gradually decreased than those at admission, and the levels of CD3+and CD4+were gradually increased than those at admission, the difference was statistically significant(P<0.05). By generalized estimating equation analysis, with admission as reference and IL-19 as covariate, CD3+and CD4+of COPD patients increased by 4.790% and 14.106% respectively on the 7th day of admission, and increased by 13.573% and 19.964% respectively on the 14th day, while CD8+decreased by 5.345%(P<0.05). IL-21 was used as a covariate, CD3+and CD4+in COPD patients increased by 6.942% and 21.275% on the 7th day after admission, and increased by 15.058% and 23.73% on the 14th day, respectively. CD8+decreased by 2.633%(P<0.05). The levels of CD3+, CD4+and CD8+T lymphocytes in COPD patients were related to the levels of IL-19 and IL21(P<0.05). Conclusion: The levels of serum IL-19 and IL-21 in COPD patients decreased gradually with the prolongation of treatment time, and the expression levels of the two were negatively correlated with CD3+and CD4+, and positively correlated with CD8+.

关 键 词：慢性阻塞性肺疾病 免疫功能 白细胞介素-19 白细胞介素-21 

分 类 号：R563.5[医药卫生—呼吸系统]