氮限制条件下链状亚历山大藻H3K4me3的调控机制解析  

Studies on the Regulation Mechanism of H3K4me3 in Alexandrium pacificum Under Nitrogen Restriction

作　　者：祁娟 隋正红[1] 刘源[1] 朱智梅 Qi Juan;Sui Zhenghong;Liu Yuan;Zhu Zhimei(The Key Laboratory of Marine Genetics and Breeding,Ministry of Education,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋生物遗传学与育种教育部重点实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2023年第4期43-53,共11页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(41676091,41176098)资助。

摘　　要：为探索链状亚历山大藻(Alexandrium pacificam)在低氮条件下的适应性反应机制,本研究采用结合位点分析法(ChIP-seq)比较了链状亚历山大藻氮限制和氮正常条件下组蛋白第三亚基四号赖氨酸的三甲基化(H3K4me3)修饰情况及调控机制,GO分析结果表明H3K4me3修饰在低氮条件下调控了跨膜转运蛋白活性,表明在低氮条件下链状亚历山大藻增强了转运体活性来获取外界营养物质。通过KEGG分析发现还富集了植物病原菌相互作用途径和谷胱甘肽代谢途径,表明链状亚历山大藻在低氮条件下,病原菌防御和抗氧化应激对其生存至关重要。此外还发现在低氮条件下H3K4me3调控了泛素介导的蛋白质降解和自噬途径来缓解氮的耗竭而响应氮胁迫。Alexandrium pacificum is a causative species of marine harmful algal blooms.However,the mechanism of their adaptation to changes in environmental nitrogen have been poorly understood.In this study,ChIP-seq technology was used to compare H3K4me3 modification and its regulatory mechanism under nitrogen restriction and normal nitrogen conditions.It was suggested by GO analysis that transmembrane transporter activity was regulated by H3K4me3 modification under low nitrogen conditions,indicating that under low nitrogen conditions,transporter activity was enhanced to obtain external nutrients.KEGG analysis also revealed enrichment of the plant pathogen interaction pathways and glutathione metabolism pathways,suggesting that pathogen defense and antioxidant stress are crucial to the survival of A.pacificum under low nitrogen conditions.In addition,it was found that under low nitrogen conditions,ubiquitin-mediated protein degradation and autophagy pathways were regulated to alleviate nitrogen depletion and respond to nitrogen stress.

关 键 词：链状亚历山大藻 氮胁迫 组蛋白第三亚基四号赖氨酸的三甲基化 结合位点分析法 表观遗传学 组蛋白修饰 

分 类 号：S917.3[农业科学—水产科学] K826.15[历史地理—历史学]