高盐条件下pH对同步短程硝化反硝化脱氮性能及微生物群落的影响  

Effects of pH on Simultaneous Partial Nitrification and Denitrification Performance and Microbial Community under High Salinity Conditions

作　　者：李萌 刘如玲 陈进进 彭秋瑜 刘健[1] 佘宗莲[1] Li Meng;Liu Ruling;Chen Jinjin;Peng Qiuyu;Liu Jian;She Zonglian(The Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;Qingdao Haibohe Sewage Treatment Plant,Qingdao 266005,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [2]青岛海泊河污水处理厂,山东青岛266005

出　　处：《中国海洋大学学报（自然科学版）》2023年第4期67-76,共10页Periodical of Ocean University of China

基　　金：中央高校基本科研业务费专项项目(201964003)资助。

摘　　要：本研究采用复合序批式生物膜反应器(HSBBR)处理高盐废水,考察了进水pH对反应器性能和微生物群落结构的影响。进水pH值由7.8降至6.0和5.0以及由5.0恢复到7.8,对污染物去除效能和稳定性影响较小,COD、NH^(+)_(4)-N和总无机氮的平均去除率保持在96%、99%和91%以上。进水pH改变对NO^(-)_(2)-N积累产生明显影响,pH为7.8条件下周期内NO^(-)_(2)-N最大积累量为7.07~8.15 mg·L^(-1),明显高于pH值为6.0和5.0时的值(4.81和2.23 mg·L^(-1))。进水pH由5.0提升至7.8悬浮污泥和生物膜的微生物多样性均降低。进水pH为5.0时,反应器内相对丰度前三位的菌属为反硝化菌Defluviicoccus(14.7%~27.3%)和Candidatus_Competibacter(22.7%~26.0%)及聚磷菌Candidatus_Accumulibacter(11.8%~17.8%);pH提升至7.8时,丰度为前三位的菌属是Candidatus_Accumulibacter(7.6%~40.5%)、Candidatus_Competibacter(21.3%~26.2%)和unidentified_Spirochaetaceae(2.5%~8.7%)。进水pH从5.0升高至7.8,氨氧化菌属Nitrosomonas的相对丰度由3.3%~3.8%下降到1.2%~1.3%,反硝化菌属总丰度从43.5%~52.6%下降到24.9%~33.1%;表明低pH条件下较高丰度氨氧化菌和反硝化菌的共同作用为同步短程硝化反硝化脱氮提供了保障。In this study,a hybrid sequencing batch biofilm reactor(HSBBR) was used to treat high salinity wastewater,and the effects of influent pH on reactor performance and microbial community structure were investigated.The influent pH value decreased from 7.8 to 6.0 and 5.0 and recovered from 5.0 to 7.8,which had little influence on the removal efficiency and stability.The average removal efficiencies of COD,NH^(+)_(4)-N and total inorganic nitrogen remained above 96%,99% and 91%.The maximum accumulation of NO^(-)_(2)-N was 7.07~8.15 mg·L^(-1) when pH value was 7.8,which was significantly higher than that when pH value was 6.0 and 5.0~4.81 and 2.23 mg·L^(-1)).The microbial diversity of suspended sludge and biofilm decreased when influent pH increased from 5.0 to 7.8.When the influent pH was 5.0,the top three bacteria genera in relative abundance in the reactor were Defluviicoccus(14.7%~27.3%),Candidatus_Competibacter(22.7%~26.0%) and Candidatus_Accumulibacter(11.8%~17.8%).When the pH was raised to 7.8,the top three genera in abundance were Candidatus_Accumulibacter(7.6%~40.5%),Candidatus_Competibacter(21.3%~26.2%) and unidentified_Spirochaetaceae(2.5%~8.7%).When influent pH increased from 5.0 to 7.8,the relative abundance of Nitrosomonas decreased from 3.3%~3.8% to 1.2%~1.3%,and the total abundance of denitrifying bacteria decreased from 43.5%~52.6% to 24.9%~33.1%.The results showed that the high abundance of ammonia oxidizing bacteria and denitrifying bacteria provided a guarantee for simultaneous partial nitrification and denitrification under low pH conditions.

关 键 词：高盐 HSBBR PH 同步短程硝化反硝化 微生物群落 

分 类 号：X703[环境科学与工程—环境工程]