一株红树林真菌Phoma herbarum L28的代谢产物  

The metabolites of a mangrove fungus Phoma herbarum L28

作　　者：黎子洋 黄诺儿 黄麒森 曾美莹 杨媛 佘志刚[2] 李春远 丁唯嘉 LI Ziyang;HUANG Nuoer;HUANG Qisen;ZENG Meiying;YANG Yuan;SHE Zhigang;LI Chunyuan;DING Weijia(Key Laboratory for Biobased Materials and Energy of Ministry of Education/College of Materials and Energy,South China Agricultural University,Guangzhou 510642,China;School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]生物基材料与能源教育部重点实验室/华南农业大学材料与能源学院,广东广州510642 [2]中山大学化学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）（中英文）》2023年第2期60-65,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省基础与应用基础研究基金(2019B1515210023,2020A1515010325);广东省自然科学基金(2018A030313582);华南农业大学2022年度大学生创新创业训练项目。

摘　　要：研究了内生真菌草茎点霉Phoma herbarum L28的次级代谢产物。该菌纯化自红树林植物苦槛蓝Myoporum bontioides A.Gray,通过大米发酵培养。采用色谱层析、波谱分析等技术分离鉴定了10个单体化合物fusarihexin A(1),fusarihexin B(2),botryosphaerihydrofuran(3),N1-methyl-2-pyridone-5-carboxamide(4),4′,5,6-trihydroxy-7-methoxyisoflavone(5),ascomfuran A(6),4-羟基苯乙酸(7),protocatechuic acid(8),胸腺嘧啶脱氧核苷(9),nortetillapyrone(10)。其中化合物1,2,3,4,5,10为首次从Phoma属中分离获得。二倍稀释法抗菌测试显示化合物1,2对O78血清型大肠杆菌Escherichia coli(禽致病性),化合物3和10对小麦赤霉菌Fusarium graminearum,化合物4对番茄枯萎菌Fusarium oxysporum显示强抗菌活性。The study investigated the metabolites of an endophytic fungus Phoma herbarum L28 derived from the mangrove plant Myoporum bontioides A.Gray.The fungus was fermented in the rice medium.Ten monomeric compounds were isolated and identified as fusarihexin A(1),fusarihexin B(2),botryosphaerihydrofuran(3),N1-methyl-2-pyridone-5-carboxamide(4),4′,5,6-trihydroxy-7-methoxyisoflavone(5),ascomfuran A(6),4-hydroxyphenylacetic acid(7),protocatechuic acid(8),thymidine(9)and nortetillapyrone(10)by the modern chromatography technology and spectral analysis.Among them,compounds 1,2,3,4,5 and 10 were firstly isolated from Phoma sp.The activity bioassay via the double dilution method showed that the antimicrobial activities of following compounds were strong,they are compounds 1 and 2 against Escherichia coli with O78 serotype,compounds 3 and 10 against Fusarium graminearum,and compound 4 against Fusarium oxysporum respectively.

关 键 词：苦槛蓝 草茎点霉 次级代谢产物 抗菌活性 

分 类 号：O629.9[理学—有机化学]