聚乳酸-羟基乙酸共聚物微球在骨组织工程中的应用  

Application of poly(lactic-co-glycolic acid)copolymer microspheres in bone tissue engineering

作　　者：张晓宇 陈琪 杨兴 郝跃峰[1] Zhang Xiaoyu;Chen Qi;Yang Xing;Hao Yuefeng(Affiliated Suzhou Hospital of Nanjing Medical University,Suzhou 215000,Jiangsu Province,China)

机构地区：[1]南京医科大学附属苏州医院,江苏省苏州市215000

出　　处：《中国组织工程研究》2023年第30期4896-4903,共8页Chinese Journal of Tissue Engineering Research

基　　金：江苏省科学技术厅自然科学基金面上项目(BK20211083),项目负责人:郝跃峰;苏州市医学重点学科项目(SZXK202111),项目负责人:郝跃峰。

摘　　要：背景:聚乳酸-羟基乙酸共聚物微球具有良好的生物相容性、生物安全性和生物降解性,作为载体已经被广泛应用于骨组织工程中,但其仍有缺乏亲水性、副产物酸性及缺乏功能化等缺点,因此需要进行不同的修饰改性方能更好地应用。目的:对聚乳酸-羟基乙酸共聚物微球在骨组织工程中的应用进行综述。方法:以“poly(lactic-co-glycolic acid)copolymer microsphere,PLGA microsphere,growth factor,drug deliver,modified,functional modification,composite scaffold,bone tissue engineering”为检索词在Web of Science和PubMed数据库检索自2000年1月至2022年6月收录的相关文献,并将其进行筛选和分析,最终选择83篇文献进行综述。结果与结论:①聚乳酸-羟基乙酸共聚物微球的理化性质与其乳酸和羟基乙酸比、分子质量和端基团等因素有关。目前,乳酸和羟基乙酸比例为75∶25、相对分子质量为75000-100000,端羧基的聚乳酸-羟基乙酸共聚物在制备微球时应用较多。②现在常用的制备方法主要包括乳化法、微流控技术、电喷雾、喷雾干燥和超临界流体法。具体的制备方法需结合微球的应用需求及生产条件来选择,随着技术的成熟与进步,未来会有更稳定、高效的生产方式。③聚乳酸-羟基乙酸共聚物微球的修饰改性包括负载生长因子和药物、功能化修饰以及以微球为基础构建复合支架。负载生长因子可促进细胞分化、血管生成,从而促进骨组织的修复与再生;负载药物可治疗多种骨骼类疾病;复合无机矿物可以提高聚乳酸-羟基乙酸共聚物的力学性能,而且提供骨组织生长所需的微量元素,更多的被用于骨缺损的治疗;通过增加微球调控细胞的分泌与活性的能力,参与疾病的免疫调控,为疾病治疗提供新思路;以聚乳酸-羟基乙酸共聚物微球为基础的复合支架,在支架原有的优点外,还能增加微创递送药物、缓释药物的能力,�BACKGROUND:Poly(lactic-co-glycolic acid)copolymer microspheres have good biocompatibility,biosafety and biodegradability.They are widely used in bone tissue engineering as a carrier.However,they still have some shortcomings,such as a lack of hydrophilicity,the acidity of by-products,lack of functionalization,etc.Therefore,different modifications are needed for better application.OBJECTIVE:To review the application of poly(lactic-co-glycolic acid)copolymer microspheres in bone tissue engineering.METHODS:With“poly(lactic-co-glycolic acid)copolymer microsphere,PLGA microsphere,growth factor,drug delivery,modified,functional modification,composite scaffold,bone tissue engineering”as the search terms in English,the original articles collected from January 2000 to June 2022 in Web of Science and PubMed databases were searched,and screened and analyzed.Finally,83 articles were selected for review.RESULTS AND CONCLUSION:(1)The physical and chemical properties of poly(lactic-co-glycolic acid)microspheres are related to the ratio of lactic acid to glycolic acid,molecular weight and terminal groups.At present,the ratio of lactic acid to glycolic acid is 75:25,the molecular weight is 75000-100000,and the carboxyl terminated poly(lactic-co-glycolic acid)copolymer is widely used in the preparation of microspheres.(2)Now the commonly used preparation methods mainly include emulsification,microfluidic technology,electric spray,spray drying and supercritical fluid method.The specific preparation methods should be selected according to the application requirements and production conditions of microspheres.With the development of technology,there will be a more stable and efficient production method.(3)The modification of poly(lactic-co-glycolic acid)copolymer microspheres includes the loading of growth factors and drugs,functional modification and the construction of composite scaffolds based on microspheres.Loading growth factors can promote cell differentiation and angiogenesis,thus promoting the repair and regeneration of b

关 键 词：聚乳酸-羟基乙酸共聚物微球 生长因子 药物递送 改性 功能化修饰 复合支架 骨组织工程 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364