干旱胁迫下玉米叶片活性氧及其清除酶的研究  

Study of Reactive Oxygen Species and Corresponding Scavenging Enzymes of Maize Leaves under Drought Stress

作　　者：张瑞益 史雷[1] 刘寰 ZHANG Ruiyi;SHI Lei;LIU Huan(College of Life Sciences,Yulin University,Yulin,Shaanxi 719000,China)

机构地区：[1]榆林学院生命科学学院,陕西榆林719000

出　　处：《陕西农业科学》2023年第2期39-43,共5页Shaanxi Journal of Agricultural Sciences

基　　金：项目编号:陕北沙区青贮玉米新品种引进及配套丰产栽培技术研究示范,2022NY-096。项目类别:陕西省科学技术厅一般项目—农业领域。

摘　　要：试验以郑单958玉米为材料,运用盆栽控水的方式,通过生理指标测定和细胞学染色观察相结合的方法,对轻度水分亏缺处理、中度水分亏缺处理、重度水分亏缺处理、对照组(土壤含水量分别为田间最大持水量的45%~55%、55%~65%、65%~75%、75%~85%)这四种水分处理下苗期玉米幼苗叶片进行活性氧代谢、分布及其清除酶系统的研究。结果表明:随着水分亏缺程度的加重,超氧阴离子自由基(O^(2-)),H_(2)O_(2),MDA含量及POD活性均升高;而SOD、CAT活性随水分亏缺程度的加重呈先上升再下降的趋势。叶表皮中H_(2)O_(2)的含量随水分亏缺程度的加重呈增加趋势,且在重度水分亏缺处理下分布较其它试验组情况更广。试验为玉米抗旱机理的研究提供了实验依据,同时也就解决旱区玉米种植问题提供了一定的理论指导。The effects of the physiological indexes and the distribution of hydrogen peroxide free radicals on maize seedlings growth were studied under the condition of controlling water in pot experiments.Zhengdan 958 was used as test material,four treatments,including water deficit(45%~55%water-holding capacity),moderate water deficit(55%~65%water-holding capacity),severe water deficit(65%~75%water-holding capacity)and control(75%~85%water-holding capacity),were set to study the reactive oxygen metabolism,distribution and its and scavenger enzyme system.The results showed that the contents of superoxide radical,hydrogen peroxide and MDA increased with increase of the water deficit,and the activity of POD had the same trend.While the activities of SOD and CAT were different from them,it was on rising trend at first,and then on declining trend.The content of H_(2)O_(2)in leaf epidermis increased with the severity of water deficit,and it was more widely distributed under severe water deficit treatment than other experimental groups.This study provides an experimental basis for the mechanism of maize resistance,and the theoretical guidance for solving the problems in maize of arid area.

关 键 词：干旱胁迫 活性氧 保护酶系统 

分 类 号：S[农业科学]