柱花草响应炭疽菌侵染的差异磷酸化蛋白质组学分析  

Differential Phosphoproteomic Analysis of Stylosanthes in Response to Colletotrichum gloeosporioides

作　　者：张世子 杨丽云 高静[1] 王芳[1] 蒋凌雁 ZHANG Shi-zi;YANG Li-yun;GAO Jing;WANG Fang;JIANG Ling-yan(College of Tropical Crops,Hainan University/Key Laboratory of Sustainable Utilization of Tropical Biological Resources of Hainan Province,Haikou,Hainan Province 570228,China)

机构地区：[1]海南大学热带作物学院/海南省热带生物资源可持续利用重点实验室,海南海口570228

出　　处：《草地学报》2023年第3期699-709,共11页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(31960342);中国科协青年人才托举工程第六届项目(2020QNRC001);海南省科协青年科技英才创新计划项目(QCXM202001)资助。

摘　　要：磷酸化是重要的蛋白翻译后修饰,在调节植物免疫方面起重要作用,但目前对柱花草与炭疽菌互作的磷酸化知之甚少。本文以太空诱变柱花草抗病株系‘2001-84’和感病株系‘2001-71’为试验材料,通过非标记定量磷酸化蛋白质组学,分析了两种株系接种炭疽菌前后的差异磷酸化蛋白。结果表明:在‘2001-84’和‘2001-71’中分别有138个和106个蛋白的磷酸化丰度特异性响应炭疽菌侵染。蛋白功能与代谢通路富集分析发现两者具有明显的差异:‘2001-84’特异性磷酸化蛋白主要定位在质膜附近,参与胞内信号传导,并显著富集在MAPK级联信号通路、植物激素信号传导、病原菌互作途径等代谢通路;‘2001-71’则更多的定位于细胞器膜上,参与细胞器的分解,代谢通路只在剪接体途径显著富集。通过对‘2001-84’特异性磷酸化蛋白进一步分析,发掘了跟抗病相关的蛋白。本文研究可为后续解析柱花草抗炭疽病的分子机制和培育柱花草抗病品种提供参考。Phosphorylation is an important post-translational modification of protein,which plays an important role in regulating plant immunity.However,little is known about the phosphorylation in the interaction between Stylosanthes spp.(stylo)and Colletotrichum gloeosporioides.In this study,label-free based phosphoproteomics technology was used to study the phosphoproteome change of disease-susceptible stylo line‘2001-71’and resistant line‘2001-84’infected with C.gloeosporioides,which were created by space flight mutation.It had been identified out 138 and 106 differentially phosphorylated proteins(DPPs)in‘2001-84’and‘2001-71’respectively in response to C.gloeosporioides infection specifically.By the analysis of protein function of DPPs and enrichment analysis of metabolic pathway,it was found out a significant difference existed between the resistant and susceptible lines.The specific DPPs in the line‘2001-84’were mainly located around the plasma membrane,involving into the intracellular signal transduction,and significantly enriched in the MAPK cascade signaling,plant hormone signaling and pathogen interaction pathway checked out by metabolic pathway analysis.The specific DPPs in the line‘2001-71’were mainly localized in organelle membrane,involving in organelle decomposition,and only significantly enriched in the spliceosome pathway.Further analysis on the specific DPPs in the line‘2001-84’,some proteins relevant to disease resistance had been uncovered.This study could provide a basis for further analysis on the resistance mechanisms in stylo to the anthracnose and breeding resistant cultivars of stylo.

关 键 词：柱花草 胶胞炭疽菌 磷酸化蛋白质组学 抗病蛋白 

分 类 号：Q946.1[生物学—植物学]