碧护缓解低温对草坪草萌发及苗期胁迫的作用  

Effects of VitaCat on Alleviating Low Temperature Stress to Turfgrass Species at Germination and Seedling Growth Stage

作　　者：李震 戴玲玲 邓海玲 郭海滨 强胜 宋小玲 LI Zhen;DAI Ling-ling;DENG Hai-ling;GUO Hai-bin;QIANG Sheng;SONG Xiao-ling(Weed Research Laboratory,college of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu Province 210095,China;Nanjing Shengjia Tian Biotechnology Co.,Ltd,Nanjing,Jiangsu Province 210095,China)

机构地区：[1]南京农业大学生命科学学院杂草研究室,江苏南京210095 [2]南京盛甲田生物科技有限公司,江苏南京210095

出　　处：《草地学报》2023年第3期804-812,共9页Acta Agrestia Sinica

基　　金：南京农业大学杂草研究室与南京盛甲田生物科技有限公司合作项目(RD01-2023)资助。

摘　　要：为探明碧护(0.136%赤·吲乙·芸苔可湿性粉剂)对草坪草种子和幼苗低温胁迫的缓解作用,以高羊茅(Festuca arundinacea Schreb.)和多年生黑麦草(Lolium perenne L.)为供试材料,研究两种草坪草种子在碧护2500~15000稀释倍液浸种24 h后,置于常温(25℃/25℃)和低温(15℃/10℃)下的种子萌发情况,观察两种草坪草幼苗期叶面喷施碧护2500~15000稀释倍液后,置于常温(25℃)和连续10 d户外低温(11℃/-5℃)下的生长情况。结果表明:两种草坪草种子在碧护7500~10000倍液浸种后均能显著提高其在常温和低温胁迫下的发芽势、发芽率、芽长及根长。低温胁迫前叶面喷施碧护5000~7500倍液后,两种草坪草的根长、根重及叶绿素含量、脯氨酸含量和超氧化物歧化酶活性显著提高,丙二醛含量和相对电导率显著降低。综上所述,碧护7500~10000倍液浸种后可提高常温和低温条件下草坪草种子萌发能力,叶面喷施碧护5000~7500倍可缓解低温胁迫对幼苗的伤害,提高其抗寒能力。In order to study the alleviating effect of VitaCat(GA·IAA·BR 0.136%WP)on seed germination and seedling growth of turfgrass under low temperature stress,two turfgrass species,Festuca arundinacea and Lolium perenne were used as the experiment material.The seeds were soaked into 2500~15000 times VitaCat for 24 hours at room temperature(25℃/25℃),then their germination were observed under room temperature(25℃/25℃)and low temperature(15℃/10℃).The seedling were foliar-applied with 2500~15000 times VitaCat,then their growth performance was observed under room temperature(25℃)and 10-day outdoor low temperature stress.The results showed that seed germination potential,seed germination rate and sprout length and root length of two turfgrass species under both low and room temperatures were significantly improved after the soaking-up in 7500~10000 times dilution of VitaCat.The root length,root weight,as well as chlorophyll content,proline content and superoxide dismutase activity of those turfgrasses were significantly increased,and malondialdehyde content and relative electrical conductivity were significantly decreased under low temperature stress by spraying 5000~7500 times VitaCat.From the above results,it can be concluded the seed soaking-up with 7500~10000 times dilution of VitaCat improved the seed germination and growth of two turfgrasses.The foliar spraying of 5000~7500 times dilution of VitaCat alleviated the injury of seedlings by low temperature stress and enhanced the cold resistance of seedlings.

关 键 词：碧护 低温胁迫 高羊茅 多年生黑麦草 种子萌发 生理指标 

分 类 号：S945.34[农业科学—水产养殖]