变灰青霉BIBA-G563对紫花苜蓿镰刀菌根腐病的防效  

Prevention Effect of Penicillium canescens BIBA-G 563 on Fusarium Root Rot of Alfalfa

作　　者：任乃芃 刘香萍[1] 唐佳琳 董晨旭 白贞婷 轩一芙 李孟滕 REN Naipeng;LIU Xiangping;TANG Jialin;DONG Chenxu;BAI Zhenting;XUAN Yifu;LI Mengteng(School of Animal Science and Technology,Heilongjiang Bayi Agricultural Reclamation University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国草地学报》2023年第3期95-103,共9页Chinese Journal of Grassland

基　　金：黑龙江省科技计划省院科技合作项目(YS20B10);大庆市新能源领域“揭榜挂帅”科技攻关项目(2021BD05);黑龙江八一农垦大学研究生创新科研项目(YJSCX2021-Y01);黑龙江八一农垦大学学成、引进人才科研启动计划项目(XYB201813);黑龙江八一农垦大学三横三纵支持计划项目(ZRCPY201908)。

摘　　要：从黑龙江省安达市黑龙江八一农垦大学草业科学系试验田健康紫花苜蓿根部分离出1株拮抗真菌BIBA-G563,对其进行形态学观察和分子生物学鉴定,并研究其对紫花苜蓿根腐病病原真菌镰刀菌的抑制能力及盆栽防治根腐病的效果。经鉴定,拮抗真菌BIBA-G563为变灰青霉。BIBA-G563与腐皮镰刀菌、尖孢镰刀菌、层出镰刀菌、轮枝镰刀菌四种病原真菌的第9 d平板对峙抑制率在28.62%~64.67%之间,无菌发酵液对四种病原菌菌丝生长的抑制率在10.34%~15.98%之间,对四种病原菌孢子萌发的抑制率在16.95%~35.95%之间,对盆栽紫花苜蓿镰刀菌根腐病防效为75.40%。表明生防真菌BIBA-G563能够有效抑制紫花苜蓿根腐病病原菌,具有良好的开发前景。In this paper,a rhizosphere fungus strain BIBA-G563 was isolated from the root of healthy alfalfa in the experimental field of Department of Prataculture Science of Heilongjiang Bayi Agricultural University,and its morphological observation and molecular biological identification were conducted.The inhibition ability of the BIBA-G563 to the pathogenic fungus Fusarium spp.of alfalfa root rot,and its effect of pot culture on the prevention and treatment of root rot were carried out.The antagonistic fungus BIBA-G563 was identified as Penicillium canescens.The results showed that inhibition rates on the 9th day of BIBA-G563 on four pathogenic fungi,F.solani,F.oxysporum,F.proliferatum and F.verticillioide,were 28.62%-64.67%.Inhibition rates of sterile fermentation broth on mycelium growth of four pathogens were 10.34%-15.98%.Inhibition rates of sterile fermentation broth on spore germination of four pathogens were 16.95%-35.95%,and the control efficiency of strain BIBA-G563 against Fusarium root rot of potted alfalfa was 75.40%.It showed that biocontrol fungus BIBA-G563 could effectively inhibit the pathogens of alfalfa root rot,and had a good development prospect for the control of Fusarium root rot of alfalfa.

关 键 词：紫花苜蓿 变灰青霉 根腐病 镰刀菌 生物防治 

分 类 号：S812.6[农业科学—草业科学]