香蕉叶斑病病原菌芭蕉新拟盘多毛孢的鉴定  

Identification of the Neopestalotiopsis musae Pathogen of Banana Leaf Spot Disease

作　　者：梁嘉莉 吴启松[1] 陈广全 张荣[1,3] 徐春香[1] 冯淑杰[1,3] LIANG Jiali;WU Qisong;CHEN Guangquan;ZHANG Rong;XU Chunxiang;FENG Shujie(College of Horticulture,South China Agricultural University,Guangzhou 510642,China;Maoming Agricultural Technology Extension Center,Maoming,Guangdong 525000,China;Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Integrative Microbiology Research Centre,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学园艺学院,广州510642 [2]茂名市农业科技推广中心,广东茂名525000 [3]华南农业大学广东省群体微生物研究中心,广州510642

出　　处：《园艺学报》2023年第2期410-420,共11页Acta Horticulturae Sinica

基　　金：广东现代农业产业技术体系岗位专家项目(2019KJ109)。

摘　　要：在广东省茂名市香蕉产区进行病害调查时发现一种为害较为严重的香蕉新发叶斑病害。根据柯赫氏法则对其进行病原菌分离及致病性检测,并根据形态学特征和ITS、β-tubulin和tef1序列分析对该病原菌进行鉴定。研究结果表明:分离得到的病原菌菌株MM3-2z9能够侵染香蕉叶片,并能诱发与田间类似的症状。其菌落在PDA培养基上呈白色,产生明显的同心轮纹;分生孢子纺锤形,具4个隔膜,中间3个细胞异色,顶细胞无色,锥形,附属丝2~4根(多为3根),基部细胞有1根附属丝,中生,无色。序列同源性分析显示MM3-2z9菌株3个单孢菌株的ITS、β-tubulin和tef1序列与芭蕉新拟盘多毛孢(Neopestalotiopsis musae)菌株相应序列的一致性都高于98%,3片段串联构建系统发育树的结果表明,菌株MM3-2z9与芭蕉新拟盘多毛孢(N.musae)亲缘关系最近。基于形态学与系统发育树分析结果,鉴定菌株MM3-2z9为芭蕉新拟盘多毛孢(N.musae)。该病原菌还可快速侵染大蕉和粉蕉‘粉杂1号’的叶片。A new emerging banana leaf spot disease was found during a disease survey in the banana-producing area of Maoming City,Guangdong Province.The pathogen was isolated and tested for pathogenicity according to Koch’s postulate,and the pathogen was identified based on morphological characteristics and sequence analyses of ITS,β-tubulin and tef1 genes.The results showed that strain MM3-2z9 could infect banana leaves and induce similar symptoms as in the field.The colony of strain MM3-2z9 on the PDA medium was white,producing obvious concentric ring patterns.The conidia were fusoid with 4-septate,three median cells versicoloured,apical cell hyaline,pyramidal,with two to four filiform apical appendages(mostly three),and basal cell with appendage single was centric and hyaline.Sequence homology analysis indicated that the ITS,β-tubulin and tef1 sequences of three single-conidial strains of MM3-2z9 were more than 98%identical to the corresponding sequences of Neopestalotiopsis musae stains.The phylogenetic tree constructed by the three fragments showed that strain MM3-2z9 was most closely related to N.musae.Therefore,strain MM3-2z9 was identified as N.musae based on morphological analysis and phylogenetic tree analysis.In addition to damaging banana(Musa AAA Cavendish)leaves,the pathogen could also quickly infect the leaves of M.×paradisiaca ABB and Musa ABB Pisang Awak’Fenza 1’.

关 键 词：香蕉 叶斑病 病原菌鉴定 芭蕉新拟盘多毛孢 

分 类 号：S668.1[农业科学—果树学]