长链非编码RNA HOTAIR对HaCaT细胞上皮间质转化的研究  

Effects of HOTAIR on epithelial mesenchymal transition in HaCaT cells

作　　者：陈志雄 王晨羽 张明子[3] 刘志飞[3] 王晓军 Chen Zhixiong;Wang Chenyu;Zhang Mingzi;Liu Zhifei;Wang Xiaojun(Department of Plastic Surgery,the Second Affiliated Hospital of Nanchang University,Nanchang 330000,China;Department of Plastic Surgery,Shanghai Ninth People's Hospital,Shanghai 200011,China;Department of Plastic Surgery,Peking Union Medical College Hospital,the Chinese Academy of Medical Science,Beijing 100730,China)

机构地区：[1]南昌大学第二附属医院整形美容科,南昌330000 [2]上海市第九人民医院整复外科,上海200011 [3]中国医学科学院北京协和医院整形外科,北京100730

出　　处：《中华医学美学美容杂志》2023年第1期54-58,共5页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：北京市自然科学基金(7162156)。

摘　　要：目的:探讨LncRNA HOTAIR对低氧条件下,HaCaT细胞上皮间质转化的影响。方法:2018年1—12月,在中国医学科学院北京协和医院将HaCaT细胞分成常氧组(A组)、低氧组(B组)、sh-control+低氧组(细胞转染sh-control低氧培养,C组)、sh-HOTAIR+低氧组(细胞转染sh-HOTAIR低氧培养,D组)4个组培养。培养36 h后显微镜下观察细胞形态变化,用qPCR、蛋白质印迹法检测4个组细胞上皮钙黏素和波形蛋白表达,用Transwell法检测细胞迁移情况。结果:qPCR结果,A、B、C、D 4个组上皮钙黏素相对表达量分别为3.076±0.271、1.000±0.089、1.024±0.222、2.595±0.085;波形蛋白相对表达量分别为1.002±0.183、4.170±0.279、4.111±0.477、2.412±0.134。蛋白质印迹结果,4个组上皮钙黏素相对表达量分别为5.748±0.778、0.960±0.263、1.380±0.205、3.026±0.700;波形蛋白相对表达量分别为1.043±0.144、4.074±0.459、4.429±0.530、2.654±0.440。Transwell结果4个组细胞迁移数分别为32.700±3.930、125.400±6.260、120.100±6.790、58.24±7.06。结论:低氧下,上皮细胞发生上皮间质转化,降低LncRNA HOTAIR表达能够抑制上皮间质转化。HOTAIR参与低氧下上皮细胞上皮间质转化过程。Objective To verify that hypoxia induces epithelial mesenchymal transition in HaCaT cells,and to observe the effect of LncRNA HOTAIR on epithelial mesenchymal transition in HaCaT cells.Methods From Jan.2018 to Dec.2018 in Chinese Academy of Medical Science,HaCaT cells were divided into four groups:group A cultured under normoxia,group B cultured under hypoxia,group C transfected with sh-control cultured under hypoxia,and group D transfected with sh-LncRNA HOTAIR cultured under hypoxia.After 36 hours of culturing,the expression of E-cadherin and vimentin were detected using qPCR and Western blot.The migration of HaCaT cells was evaluated by Transwell assay.Results The relative quantity of E-cadherin mRNA in the four groups were 3.076±0.271,1.000±0.089,1.024±0.222,and 2.595±0.085,while vimentin mRNAs were 1.002±0.183,4.170±0.279,4.111±0.477,and 2.412±0.134,respectively.In addition,the Transwll invasion assay showed that numbers of cell migration in the four groups were 32.70±3.93,125.40±6.26,120.10±6.79,and 58.24±7.06,respectively.Conclusions The study suggests that hypoxia promotes epithelial mesenchymal transition in keratinocytes.Furthermore,downregulation of HOTAIR is noted to inhibit epithelial mesenchymal transition of keratinocytes under hypoxia condition.

关 键 词：瘢痕疙瘩 上皮细胞 长链非编码RNA HOTAIR 上皮间质转化 

分 类 号：R622[医药卫生—整形外科]